Predictive value of skin prick tests using recombinant allergens for diagnosis of peanut allergy

被引:181
作者
Astier, Catherine
Morisset, Martine
Roitel, Olivier
Codreanu, Fanny
Jacquenet, Sandrine
Franck, Patricia
Ogier, Virginie
Petit, Nicolas
Proust, Barbara
Moneret-Vautrin, Denise-Anne
Burks, A. Wesley
Bihain, Bernard
Sampson, Hugh A.
Kanny, Gisele
机构
[1] United Hosp, Cent Hosp, Dept Internal Med, Nancy, France
[2] Lipidomix, Lab Mol Med & Therapeut, Nancy, France
[3] Duke Univ, Med Ctr, Dept Pediat, Div Pediat Allergy & Immunol, Durham, NC USA
[4] Mt Sinai Hosp, Dept Pediat, Div Allergy & Immunol, New York, NY USA
基金
美国国家卫生研究院;
关键词
food allergy; peanut; diagnosis; recombinant allergens;
D O I
10.1016/j.jaci.2006.04.053
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Current diagnosis of peanut allergy relies on natural extracts that lack standardization. Recombinant DNA technology allows production of pure biochemically characterized proteins. Their usefulness for peanut allergy diagnosis is not established. Objective: This study aimed to evaluate the diagnostic value of the 3 major recombinant peanut allergens. Methods: Recombinant (r) Ara h1, rAra h2, and rAra h3 were produced according to the recommendations of good manufacturing practice for recombinant allergens. Skin prick tests (SPTs) and IgE ELISA assays were performed in 30 patients with peanut allergy and 30 control subjects without food allergy: 15 nonatopic and 15 sensitized to birch pollen. Disease severity was graded by clinical scoring. Results: All patients with peanut allergy showed positive SPT results to rAra h 2; 40% reacted with rAra h 1 and 27% with rAra h 3. No control subjects reacted with any of the recombinant allergens. Monosensitization to rAra h 2 was observed in 53% of patients. Neither SPT size nor levels of specific IgE were correlated with the disease severity. However, patients with monosensitization to rAra h 2 had a significantly lower severity score than polysensitized subjects and a lower level of specific IgE against peanut extract and rAra h2. Conclusion: Skin prick tests to individual recombinant peanut allergens appear to be a safe and effective diagnostic tool. Cosensitization to rAra h2 and rArah I and/or rAra h3 is predictive of more severe reactions. Clinical implications: Recombinant peanut allergens can be used by SPTs for diagnosis and evaluation of allergy severity.
引用
收藏
页码:250 / 256
页数:7
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