Vasopressin phosphorylates HSP27 in aortic smooth muscle cells

被引：11

作者：

Akamatsu, S

Nakajima, K

Ishisaki, A

Matsuno, H

Tanabe, K

Takei, M

Takenaka, M

Hirade, K

Yoshimi, N

Suga, H

Oiso, Y

Kato, K

Kozawa, O ^{[1
]}

机构：

[1] Gifu Univ, Sch Med, Dept Pharmacol, Gifu 5008705, Japan

[2] Gifu Univ, Sch Med, Dept Crit Care Med, Gifu 500, Japan

[3] Univ Ryukyus, Fac Med, Dept Tumor Pathol, Okinawa, Japan

[4] Nagoya Univ, Sch Med, Dept Internal Med 1, Nagoya, Aichi, Japan

[5] Aichi Human Serv Ctr, Inst Dev Res, Dept Biochem, Kasugai, Aichi, Japan

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2004年 / 92卷 / 06期

关键词：

vasopressin; phosphorylation; protein kinase; vascular; smooth muscle;

D O I：

10.1002/jcb.20148

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Administration of arginine vasopressin (AVP) time-dependently induced the phosphorylation of heat shock protein 27 (HSP27) at Ser-15 and Ser-85 in smooth muscle of aorta in vivo. The AVP-induced phosphorylation of HSP27 at Ser-15 and Ser-85 was inhibited by a V1a receptor antagonist but not by a V2 receptor antagonist. In cultured aortic smooth muscle A10 cells, AVP markedly stimulated the phosphorylation of HSP27 at Ser-15 and Ser-85. The AVP-induced phosphorylation of HSP27 was attenuated by SB203580 and PD169316, inhibitors of p38 mitogen-activated protein (MAP) kinase, but not by PD98059, a MEK inhibitor. These results strongly suggest that AVP phosphorylates HSP27 via p38 MAP kinase in aortic smooth muscle cells.

引用

页码：1203 / 1211

页数：9

共 19 条

[1] PD-098059 IS A SPECIFIC INHIBITOR OF THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE IN-VITRO AND IN-VIVO [J].