Electrospray mass spectrometry for the identification of MHC class I-associated peptides expressed on cancer cells

被引:21
作者
Bonner, PLR
Lill, JR
Hill, S
Creaser, CS
Rees, RC
机构
[1] Nottingham Trent Univ, Dept Life Sci, Nottingham NG11 8NS, England
[2] Nottingham Trent Univ, Dept Chem & Phys, Nottingham NG11 8NS, England
关键词
tandem mass spectrometry; electrospray ionisation; MHC class I peptides; tumour epitopes; peptide purification and sequencing;
D O I
10.1016/S0022-1759(02)00009-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Electrospray ionisation (ESI) mass spectrometry (MS) has been used extensively for the detection of peptides presented by major histocompatibility complex (MHC) molecules, This review focuses on the optimisation of electrospray mass spectrometry and the use of tandem mass spectrometry to sequence MHC class I peptides, We review the isolation of MHC class I peptides from the surface of cells with particular reference to tumour cells. In addition, we also discuss the advantages and disadvantages of the methods available to concentrate and fractionate the peptides prior to analysis by electrospray mass spectrometry. (C) 2002 Elsevier Science B,V. All rights reserved.
引用
收藏
页码:5 / 19
页数:15
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