The major outer membrane protein, CD, extracted from Moraxella (Branhamella) catarrhalis is a potential vaccine antigen that induces bactericidal antibodies

被引:30
作者
Yang, YP
Myers, LE
McGuinness, U
Chong, P
Kwok, Y
Klein, MH
Harkness, RE
机构
[1] Research Center, Pasteur Merieux Connaught Canada, North York, Ont. M2R 3T4
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1997年 / 17卷 / 03期
关键词
Branhamella catarrhalis; outer membrane protein; heat-modifiable protein; protein CD; vaccine;
D O I
10.1016/S0928-8244(97)00008-4
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The major outer membrane protein of Moraxella (Branhamella) catarrhalis, CD, was detergent-extracted from the bacterial cell wall and purified to homogeneity in high yields by a simple process. The purified protein appeared to exhibit immunogenic properties similar to those of native CD exposed on the surface of the bacterium. Antibodies to CD raised in mice specifically bound to intact B. catarrhalis, as determined by flow cytometry analysis. The IgG subclass distributions of anti-CD antibodies in sera from mice immunized with purified CD or with B. catarrhalis were also similar. CD was found to be antigenically conserved among a panel of B. catarrhalis isolates, as demonstrated by the consistent reactivities of mouse anti-CD antisera with a common 60 kDa protein on immunoblots. Furthermore, convalescent sera collected from patients with otitis media due to B. catarrhalis infection were found to be reactive with the CD protein by immunoblotting. Finally, the purified protein induced antibodies in guinea pigs and mice that exhibited in vitro bactericidal activity against the pathogen. Therefore, the native CD outer membrane protein represents a potentially useful antigen for inclusion in a vaccine against B. catarrhalis.
引用
收藏
页码:187 / 199
页数:13
相关论文
共 26 条
[1]   CURRENT THERAPY FOR OTITIS-MEDIA AND CRITERIA FOR EVALUATION OF NEW ANTIMICROBIAL AGENTS [J].
BLUESTONE, CD .
CLINICAL INFECTIOUS DISEASES, 1992, 14 :S197-S203
[2]   MOUSE IGG3 ANTIBODIES ARE HIGHLY PROTECTIVE AGAINST INFECTION WITH STREPTOCOCCUS-PNEUMONIAE [J].
BRILES, DE ;
CLAFLIN, JL ;
SCHROER, K ;
FORMAN, C .
NATURE, 1981, 294 (5836) :88-90
[3]   BRANHAMELLA-CATARRHALIS - AN ORGANISM GAINING RESPECT AS A PATHOGEN [J].
CATLIN, BW .
CLINICAL MICROBIOLOGY REVIEWS, 1990, 3 (04) :293-320
[4]   Evaluation of purified UspA from Moraxella catarrhalis as a vaccine in a murine model after active immunization [J].
Chen, DX ;
McMichael, JC ;
VanDerMeid, KR ;
Hahn, D ;
Mininni, T ;
Cowell, J ;
Eldridge, J .
INFECTION AND IMMUNITY, 1996, 64 (06) :1900-1905
[5]  
DOYLE WJ, 1989, PEDIATR INFECT DIS J, V8, pS45
[6]   OTITIS-MEDIA IN CHILDREN .1. THE SYSTEMIC IMMUNE-RESPONSE TO NONTYPABLE HEMOPHILUS-INFLUENZAE [J].
FADEN, H ;
BERNSTEIN, J ;
BRODSKY, L ;
STANIEVICH, J ;
KRYSTOFIK, D ;
SHUFF, C ;
HONG, JJ ;
OGRA, PL .
JOURNAL OF INFECTIOUS DISEASES, 1989, 160 (06) :999-1004
[7]   PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE MENINGOCOCCAL OUTER-MEMBRANE PROTEIN COMPLEX CONJUGATE VACCINES - IMMUNOGENICITY AND EFFICACY IN EXPERIMENTAL PNEUMOCOCCAL OTITIS-MEDIA [J].
GIEBINK, GS ;
KOSKELA, M ;
VELLA, PP ;
HARRIS, M ;
LE, CT .
JOURNAL OF INFECTIOUS DISEASES, 1993, 167 (02) :347-355
[8]   HUMAN IMMUNITY TO MENINGOCOCCUS .I. ROLE OF HUMORAL ANTIBODIES [J].
GOLDSCHNEIDER, I ;
GOTSCHLICH, EC ;
ARTENSTEIN, MS .
JOURNAL OF EXPERIMENTAL MEDICINE, 1969, 129 (06) :1307-+
[9]   A RECOMBINANT NON-FATTY ACYLATED FORM OF THE HI-PAL (P6) PROTEIN OF HAEMOPHILUS-INFLUENZAE ELICITS BIOLOGICALLY-ACTIVE ANTIBODY AGAINST BOTH NONTYPEABLE AND TYPE-B HAEMOPHILUS-INFLUENZAE [J].
GREEN, BA ;
METCALF, BJ ;
QUINNDEY, T ;
KIRKLEY, DH ;
QUATAERT, SA ;
DEICH, RA .
INFECTION AND IMMUNITY, 1990, 58 (10) :3272-3278
[10]   BRANHAMELLA-CATARRHALIS PATHOGENESIS IN SCID AND SCID/BEIGE MICE [J].
HARKNESS, RE ;
GUIMOND, MJ ;
MCBEY, BA ;
KLEIN, MH ;
PERCY, DH ;
CROY, BA .
APMIS, 1993, 101 (10) :805-810