The role of OPG/TRAIL complex in multiple myeloma -: The OPG/TRAIL complex in an in vitro osteoclastogenesis model derived from human multiple myeloma-bone disease

被引：11

作者：

Brunetti, Giacomina

Colucci, Silvia

Rizzi, Rita

Mori, Giorgio

Colaianni, Graziana

Oranger, Angela

Zallone, Alberta

Liso, Vincenzo

Grano, Maria

机构：

[1] Univ Bari, Sch Med, Dept Human Anat & Histol, I-70124 Bari, Italy

[2] Univ Bari, Sch Med, Dept Internal Med & Publ Med, Hematol Sect, I-70124 Bari, Italy

来源：

SKELETAL DEVELOPMENT AND REMODELING IN HEALTH, DISEASE, AND AGING | 2006年 / 1068卷

关键词：

multiple myeloma; osteoclast; T cells; TRAIL; OPG;

D O I：

10.1196/annals.1346.049

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Multiple myeloma (MM) is often associated with an increased osteoclast (OC) activity. Using an in vitro osteoclastogenesis model consisting of MM unstimulated and unfractionated peripheral blood mononuclear cells (PBMCs), we showed that T cells support OC formation and survival. Differently, in T cell-depleted MM PBMC cultures, exogenous macrophage-colony stimulating factor (M-CSF) and receptor activated of nuclear factor-kappa B ligand (RANKL) were necessary for osteoclastogenesis. We found RANKL, OPG, and TRAIL overexpression by fresh MM T cells. Despite high osteoprotegerin (OPG) levels, the persistence of osteoclastogenesis can be related to the formation of the OPG/TRAIL complex. Our results highlight that MM T cells support OC formation and survival, possibly involving OPG/TRAIL interaction.

引用

页码：334 / 340

页数：7

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