New molecular weight forms of arabinogalactan from Larix occidentalis

被引：15

作者：

Prescott, JH ^{[1
]}

Groman, EV ^{[1
]}

Gulyas, G ^{[1
]}

机构：

[1] ADV MAGNET INC,CAMBRIDGE,MA 02138

来源：

CARBOHYDRATE RESEARCH | 1997年 / 301卷 / 1-2期

关键词：

arabinogalactan; molecular weight; gel filtration; light scattering; immunoassay;

D O I：

10.1016/S0008-6215(97)00078-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Arabinogalactan fractions with narrow molecular weight distributions were obtained from a crude extract of Larix occidentalis by gel-filtration chromatography. Molecular weight distributions of the fractions were determined by a combination of gel filtration chromatography (GFC) with intensity (Rayleigh) light scattering. Two distinct distributions were observed, with the fractions in the lower distribution as small as 3 kDa. A sensitive immunoassay confirmed that the polysaccharide fractions were arabinogalactans. Compositional analysis revealed that the sub-9 kDa arabinogalactan fractions obtained by gel filtration of the crude extract have significantly lower galactose to arabinose ratios than the previously isolated sub-9 kDa arabinogalactans obtained from arabinogalactan (37 kDa) by chemical methods D.H. Prescott et al., Carbohydr. Res., 278 (1995) 113-128]. (C) 1997 Elsevier Science Ltd.

引用

页码：89 / 93

页数：5

共 16 条

[1]

ADAMS MF, 1973, IND GUMS, P415

[2]

STUDIES ON ARABOGALACTANS .2. FRACTIONATION OF THE ARABOGALACTAN FROM LARIX-OCCIDENTALIS NUTT A METHYLATION STUDY OF ONE OF THE COMPONENTS [J].