A global benchmark study using affinity-based biosensors

被引：72

作者：

Rich, Rebecca L. ^{[1
]}

Papalia, Giuseppe A. ^{[1
]}

Flynn, Peter J. ^{[2
]}

Furneisen, Jamie ^{[3
]}

Quinn, John ^{[4
]}

Klein, Joshua S. ^{[5
]}

Katsamba, Phini S. ^{[6
]}

Waddell, M. Brent ^{[7
]}

Scott, Michael ^{[8
]}

Thompson, Joshua ^{[9
]}

Berlier, Judie ^{[9
]}

Corry, Schuyler ^{[9
]}

Baltzinger, Mireille ^{[10
]}

Zeder-Lutzi, Gabrielle ^{[10
]}

Schoenemann, Andreas ^{[11
]}

Clabbers, Anca ^{[12
]}

Wieckowski, Sebastien ^{[13
]}

Murphy, Mary M. ^{[14
]}

Page, Phillip ^{[14
]}

Ryan, Thomas E. ^{[14
]}

Duffner, Jay ^{[15
]}

Ganguly, Tanmoy ^{[15
]}

Corbin, John ^{[16
]}

Gautam, Satyen ^{[17
]}

Anderluh, Gregor ^{[18
]}

Bavdek, Andrej ^{[18
]}

Reichmann, Dana ^{[19
]}

Yadav, Satya P. ^{[20
]}

Hommema, Eric ^{[21
]}

Pol, Ewa ^{[22
]}

Drake, Andrew ^{[23
]}

Klakamp, Scott ^{[23
]}

Chapman, Trevor ^{[24
]}

Kernaghan, Dawn ^{[25
]}

Miller, Ken ^{[25
]}

Schuman, Jason ^{[26
]}

Lindquist, Kevin ^{[26
]}

Herlihy, Kara ^{[26
]}

Murphy, Michael B. ^{[26
]}

Bohnsack, Richard ^{[27
]}

Andrien, Bruce ^{[28
]}

Brandani, Pietro ^{[29
]}

Terwey, Danny ^{[30
]}

Millican, Rohn ^{[31
]}

Darling, Ryan J. ^{[31
]}

Wang, Liann ^{[31
]}

Carter, Quincy ^{[31
]}

Dotzlaf, Joe ^{[31
]}

Lopez-Sagaseta, Jacinto ^{[32
]}

Campbell, Islay ^{[33
]}

机构：

[1] Univ Utah, Sch Med, Ctr Biomol Interact Anal, Salt Lake City, UT 84132 USA

[2] KaloBios Pharmaceut, San Francisco, CA 94080 USA

[3] Schering Plough Biopharma, Palo Alto, CA 94304 USA

[4] Nomadics, Oklahoma City, OK 73104 USA

[5] CALTECH, Pasadena, CA 91125 USA

[6] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[7] St Jude Childrens Res Hosp, Hartwell Ctr Bioinformat & Biotechnol, Memphis, TN 38105 USA

[8] Univ Zurich, CH-8057 Zurich, Switzerland

[9] Mol Probes Invitrogen, Eugene, OR 97402 USA

[10] Univ Strasbourg, F-67412 Illkirch Graffenstaden, France

[11] Merck KGaA, D-64293 Darmstadt, Germany

[12] Abbott Biores Ctr, Worcester, MA 01605 USA

[13] Univ Strasbourg, Inst Mol & Cellular Biol, F-67084 Strasbourg, France

[14] Reichert, Depew, NY 14043 USA

[15] Momenta Pharmaceut, Cambridge, MA 02142 USA

[16] XOMA US, Emeryville, CA 94608 USA

[17] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 119260, Singapore

[18] Univ Ljubljana, Dept Biol, Ljubljana 1000, Slovenia

[19] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[20] Cleveland Clin Fdn, Mol Biotechnol Core Lab, Cleveland, OH 44195 USA

[21] ThermoFisher Sci, Rockford, IL 61101 USA

[22] Biacore GE Healthcare, SE-75450 Uppsala, Sweden

[23] AstraZeneca, Hayward, CA 94545 USA

[24] GlaxoSmithKline, Neurodegenerat Res Dept, Harlow CM19 5AW, Essex, England

[25] Medimmune Inc, Gaithersburg, MD 20878 USA

[26] Biacore GE Healthcare, Piscataway, NJ 08854 USA

[27] Med Coll Wisconsin, Dept Biochem, Milwaukee, WI 53226 USA

[28] Alex Pharmaceut, Cheshire, CT 06410 USA

[29] Appl Biosyst Inc, Foster City, CA 94404 USA

[30] DiaDexus, San Francisco, CA 94080 USA

[31] Eli Lilly & Co, Indianapolis, IN 46285 USA

[32] Univ Navarra, Ctr Appl Med Res, Lab Thrombosis & Haemostasis, E-31080 Pamplona, Spain

[33] EMD Lexigen Res Ctr, Billerica, MA 01821 USA

[34] Ist Super Sanita, Dept Cell Biol & Neurosci, I-00161 Rome, Italy

[35] Inst Pasteur, Dept Struct Biol & Chem, F-75724 Paris, France

[36] Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA

[37] Pfizer Rinat Labs, San Francisco, CA 94080 USA

[38] PDL BioPharma, Fremont, CA 94555 USA

[39] Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem, San Antonio, TX 78229 USA

[40] Akubio, Cambridge CB4 0GJ, England

[41] Univ Bordeaux, Inst Europeen Chim & Biol, INSERM, U869, F-33607 Pessac, France

[42] Wyeth Res, Cambridge, MA 02140 USA

[43] Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark

[44] Novo Nordisk AS, DK-2820 Gentofte, Denmark

[45] Sodertorns Univ Coll, S-14152 Huddinge, Sweden

[46] Temple Univ, Dept Biochem, Philadelphia, PA 19140 USA

[47] US FDA, Bethesda, MD 20892 USA

[48] UCL, Dept Biochem Engn, London WC1E 7JE, England

[49] Merck, I-00040 Rome, Italy

[50] Roche, Palo Alto, CA 94304 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 386卷 / 02期

关键词：

Biacore; Kinetics; Optical biosensor; Surface plasmon resonance; RATE CONSTANTS; BIACORE; KINETICS; BINDING;

D O I：

10.1016/j.ab.2008.11.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：194 / 216

页数：23

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