Transcriptional activity and constitutive nuclear localization of the ETS protein Elf-1

被引：32

作者：

BredemeierErnst, I ^{[1
]}

Nordheim, A ^{[1
]}

Janknecht, R ^{[1
]}

机构：

[1] HANNOVER MED SCH,INST MOL BIOL,D-30623 HANNOVER,GERMANY

来源：

FEBS LETTERS | 1997年 / 408卷 / 01期

关键词：

Elf-1; ETS protein; gene expression; lymphocyte; nuclear localization; transactivation;

D O I：

10.1016/S0014-5793(97)00387-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Elf-1 is a lymphoid-specific transcription factor that belongs to the ETS protein family. It can bind to DNA target sequences within a variety of cytokine genes. We demonstrate that Elf-1 is constitutively localized in the nucleus which is dependent on the presence of amino acids 86-265. Analysis of Gal4-Elf-1 fusion proteins revealed that the N-terminal 86 amino acids of Elf-1 contain a transcriptional activation domain, the activity of which is attenuated by an internal repression domain. Furthermore, Elf-1 interacts specifically with the E74 target sequence and can stimulate transcription driven by the E74 site independent of mitogenic signaling. Thus, Elf-1 is able to stimulate gene transcription which may be required for the development and activity of lymphocytes. (C) 1997 Federation of European Biochemical Societies.

引用

页码：47 / 51

页数：5

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