TRPV4-dependent dilation of peripheral resistance arteries influences arterial pressure

被引:179
作者
Earley, Scott [1 ,2 ]
Pauyo, Thierry [1 ]
Drapp, Rebecca [1 ]
Tavares, Matthew J. [1 ]
Liedtke, Wolfgang [3 ]
Brayden, Joseph E. [1 ]
机构
[1] Univ Vermont, Dept Pharmacol, Coll Med, Burlington, VT 05405 USA
[2] Colorado State Univ, Vasc Physiol Res Grp, Dept Biomed Sci, Ft Collins, CO 80523 USA
[3] Duke Univ, Med Ctr, Ctr Translat Neurosci, Durham, NC USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2009年 / 297卷 / 03期
关键词
calcium-activated potassium channels; endothelium; vascular smooth muscle; blood pressure; epoxyeicosatrienoic acid; transient receptor potential vanilloid 4; CATION CHANNEL TRPV4; RECEPTOR POTENTIAL VANILLOID-4; CA2+-ACTIVATED K+ CHANNEL; HYPERPOLARIZING FACTOR; SMALL-CONDUCTANCE; EPOXYEICOSATRIENOIC ACIDS; ENDOTHELIAL-CELLS; ARACHIDONIC-ACID; BLOOD-PRESSURE; MICE;
D O I
10.1152/ajpheart.00241.2009
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Earley S, Pauyo T, Drapp R, Tavares MJ, Liedtke W, Brayden JE. TRPV4-dependent dilation of peripheral resistance arteries influences arterial pressure. Am J Physiol Heart Circ Physiol 297: H1096-H1102, 2009. First published July 17, 2009; doi: 10.1152/ajpheart.00241.2009. Transient receptor potential vanilloid 4 (TRPV4) channels have been implicated as mediators of calcium influx in both endothelial and vascular smooth muscle cells and are potentially important modulators of vascular tone. However, very little is known about the functional roles of TRPV4 in the resistance vasculature or how these channels influence hemodynamic properties. In the present study, we examined arterial vasomotor activity in vitro and recorded blood pressure dynamics in vivo using TRPV4 knockout (KO) mice. Acetylcholine-induced hyperpolarization and vasodilation were reduced by similar to 75% in mesenteric resistance arteries from TRPV4 KO versus wild-type (WT) mice. Furthermore, 11,12-epoxyeicosatrienoic acid (EET), a putative endothelium-derived hyperpolarizing factor, activated a TRPV4-like cation current and hyperpolarized the membrane of vascular smooth muscle cells, resulting in the dilation of mesenteric arteries from WT mice. In contrast, 11,12-EET had no effect on membrane potential, diameter, or ionic currents in the mesenteric arteries from TRPV4 KO mice. A disruption of the endothelium reduced 11,12-EET-induced hyperpolarization and vasodilatation by similar to 50%. A similar inhibition of these responses was observed following the block of endothelial (small and intermediate conductance) or smooth muscle (large conductance) K+ channels, suggesting a link between 11,12-EET activity, TRPV4, and K+ channels in endothelial and smooth muscle cells. Finally, we found that hypertension induced by the inhibition of nitric oxide synthase was greater in TRPV4 KO compared with WT mice. These results support the conclusion that both endothelial and smooth muscle TRPV4 channels are critically involved in the vasodilation of mesenteric arteries in response to endothelial-derived factors and suggest that in vivo this mechanism opposes the effects of hypertensive stimuli.
引用
收藏
页码:H1096 / H1102
页数:7
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