Disruption of ubiquitin-related genes in laboratory yeast strains enhances ethanol production during sake brewing

被引：12

作者：

Wu, Hong ^{[1
]}

Watanabe, Tomoko ^{[1
,2
]}

Araki, Yoshio ^{[1
]}

Kitagaki, Hiroshi ^{[1
]}

Akao, Takeshi ^{[1
]}

Takagi, Hiroshi ^{[3
]}

Shimoi, Hitoshi ^{[1
,2
]}

机构：

[1] Natl Res Inst Brewing, Higashihiroshima 7390046, Japan

[2] Hiroshima Univ, Grad Sch Adv Sci Matter, Higashihiroshima 7398530, Japan

[3] Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma 6300192, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2009年 / 107卷 / 06期

关键词：

Saccharomyces cerevisiae; Sake brewing; Ubiquitin; ubi4; Yeast; SACCHAROMYCES-CEREVISIAE; DEGRADATION; PERMEASE; EXPRESSION; STARVATION; RESISTANCE; TOLERANCE; TRANSPORT; PROTEINS; COMPLEX;

D O I：

10.1016/j.jbiosc.2009.01.014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sake yeast can produce high levels of ethanol in concentrated rice mash. While both sake and laboratory yeast strains belong to the species Saccharomyces cerevisiae, the laboratory strains produce much less ethanol. This disparity in fermentation activity may be due to the strains' different responses to environmental stresses, including ethanol accumulation. To obtain more insight into the stress response of yeast cells under sake brewing conditions, we carried out small-scale sake brewing tests using laboratory yeast strains disrupted in specific stress-related genes. Surprisingly, yeast strains with disrupted ubiquitin-related genes produced more ethanol than the parental strain during sake brewing. The elevated fermentation ability conferred by disruption of the ubiquitin-coding gene UBI4 was confined to laboratory strains, and the ubi4 disruptant of a sake yeast strain did not demonstrate a comparable increase in ethanol production. These findings suggest different roles for ubiquitin in sake and laboratory yeast strains. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：636 / 640

页数：5

共 26 条

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