Expression of the cutaneous lymphocyte-associated antigen in circulating T cells in drug-allergic reactions

被引:23
作者
Gonzalez, FJ [1 ]
Carvajal, MJ [1 ]
Leiva, L [1 ]
Juarez, C [1 ]
Blanca, M [1 ]
Santamaria, LF [1 ]
机构
[1] CARLOS HAYA HOSP, DEPT INTERNAL MED, RES UNIT ALLERG DIS, E-29010 MALAGA, SPAIN
关键词
T lymphocytes; allergy; cutaneous lymphocyte-associated antigen; homing; skin; humans;
D O I
10.1159/000237597
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Mechanisms underlying the production of delayed cutaneous reactions to drugs are poorly characterized. The cutaneous lymphocyte-associated antigen (CLA) is a skin-homing T cell receptor that defines T lymphocytes associated with the cutaneous immune response. We studied the percentage and activation phenotype of circulating CLA+ T cells in drug allergic patients and healthy controls. Methods: PBMCs were isolated from heparinized venous blood by Ficoll density gradient. Lymphocytes were stained for flow cytometry with anti-CLA, anti-CD3 and anti-HLA-DR mAbs. Five-parameter analysis was performed on an Ortho Cytoron Absolute flow cytometer. Results: We found increased percentages of circulating CLA+ T cells in drug-allergic patients compared to controls. Moreover, CLA+ T cells from drug-allergic individuals expressed a higher percentage of the T cell activation marker HLA-DR. Conclusions: These results suggest that CLA+ T cells may play a role in the pathology of delayed cutaneous reactions to drugs. Further studies are in progress to elucidate the role of skin-homing T cells in allergic reactions to drugs.
引用
收藏
页码:345 / 347
页数:3
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