Two methods of whole-genome amplification enable accurate genotyping across a 2320-SNP linkage panel

被引：153

作者：

Barker, DL

Hansen, MST

Faruqi, AF

Giannola, D

Irsula, OR

Lasken, RS

Latterich, M

Makarov, V

Oliphant, A

Pinter, JH

Shen, R

Sleptsova, I

Ziehler, W

Lai, E ^{[1
]}

机构：

[1] GlaxoSmithKline, Genet Res, Res Triangle Pk, NC 27709 USA

[2] Illumina Inc, San Diego, CA USA

[3] Mol Staging Inc, New Haven, CT 06511 USA

[4] Rubicon Genom, Ann Arbor, MI 48108 USA

来源：

GENOME RESEARCH | 2004年 / 14卷 / 05期

关键词：

D O I：

10.1101/gr.1949704

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comprehensive genome scans involving many thousands of SNP assays will require significant amounts of genomic DNA from each sample. We report two successful methods for amplifying whole-genomic DNA prior to SNP analysis, multiple displacement amplification, and OmniPlex technology. We determined the coverage of amplification by analyzing a SNP linkage marker set that contained 2320 SNP markers spread across the genome at an average distance of 2.5 cM. We observed a concordance of >99.8% in genotyping results from genomic DNA and amplified DNA, strongly indicating the ability of both methods used to amplify genomic DNA in a highly representative manner. Furthermore, we were able to achieve a SNP call rate of >98% in both genomic and amplified DNA. The combination of whole-genome amplification and comprehensive SNP linkage analysis offers new opportunities for genetic analysis in clinical trials, disease association studies, and archiving of DNA samples.

引用

收藏

页码：901 / 907

页数：7

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