GATEWAY™ vectors for Agrobacterium-mediated plant transformation

被引：2947

作者：

Karimi, M ^{[1
]}

Inzé, D ^{[1
]}

Depicker, A ^{[1
]}

机构：

[1] State Univ Ghent VIB, Dept Plant Syst Biol, B-9000 Ghent, Belgium

来源：

TRENDS IN PLANT SCIENCE | 2002年 / 7卷 / 05期

关键词：

D O I：

10.1016/S1360-1385(02)02251-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY(TM) conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.

引用

页码：193 / 195

页数：3

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