Mytilus edulis pedal ganglia express μ opiate receptor transcripts exhibiting high sequence identity with human neuronal μ1

Previous pharmacological and biochemical evidence suggests that mu-subtype opiate receptors are expressed in the mollusk Mytilus edulis (Bivalve), including the organism's ganglia. In this study, we present molecular evidence of mu opiate receptor expression. Using primers derived from the human neuronal mu l opiate receptor, we used reverse transcription-polymerase chain reaction (RT-PCR) to detect expression of mu transcripts from Mytilus pedal ganglia. Sequence analysis of the RT-PCR products revealed 95% identity with the neuronal human mu l receptor. Furthermore, interleukin-1 and morphine exposure to excised pedal ganglia resulted in up- and down-regulation of the mu receptor transcripts, respectively. This study provides molecular evidence that mu-type opiate receptors are expressed in molluscan ganglia, suggesting that they first appear in invertebrate organisms and are retained during evolution. (C) 1999 Elsevier Science B.V. All rights reserved.