Simultaneous determination of methadone, heroin and their metabolites in hair by GC-MS

被引:10
作者
Bermejo, AM [1 ]
Lucas, ACS
Tabernero, MJ
Fernández, P
机构
[1] Fac Med, Dept Legal Med, Forens Toxicol Serv, Santiago De Compostela, Spain
[2] Univ Amazonas, Fac Pharm, Dept Clin Anal & Toxicol, Aleixo, Brazil
关键词
GC/MS; hair; methadone; codeine; morphine; MAM; heroin;
D O I
10.1080/00032710008543087
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method for the simultaneous identification and quantitation of methadone, codeine, morphine, monoacetylmorphine and heroin in hair by use of gas chromatography in combination with mass spectrometry (GC-MS) is proposed. Hair samples are incubated with dithiothreitol and subsequently subjected to enzymatic hydrolysis with Pronase E. Following addition of their deuterated internal standards, samples are subjected to liquid-liquid extraction in ToxiTubes A (R). The residue thus obtained is derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide/trimethylchlorosilane (BSTFA/TMCS) and directly injected into a GC-MS in the single ion mode (SIM). The calibration curve is linear over the range 0.5-50 ng/mg for all the analytes. The method was reproducible with limits of detection between 0.06 ng/mg (methadone) and 0.29 ng/mg (codeine). This method was applied in a 5-years-old child suspected to be victim of a coercive drug administration.
引用
收藏
页码:739 / 752
页数:14
相关论文
共 10 条
[1]  
BAUMGARTNER WA, 1989, J FORENSIC SCI, V34, P1433
[2]  
BAUMGARTNER WA, 1990, 2 INT C THER DRUG MO
[3]   HAIR ANALYSIS - PERSPECTIVES AND LIMITS OF A PROPOSED FORENSIC METHOD OF PROOF - A REVIEW [J].
BOST, RO .
FORENSIC SCIENCE INTERNATIONAL, 1993, 63 (1-3) :31-42
[4]   TESTING HUMAN HAIR FOR DRUGS OF ABUSE .3. IDENTIFICATION OF HEROIN AND 6-ACETYLMORPHINE AS INDICATORS OF HEROIN USE [J].
GOLDBERGER, BA ;
CAPLAN, YH ;
MAGUIRE, T ;
CONE, EJ .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1991, 15 (05) :226-231
[5]   SIMULTANEOUS QUANTIFICATION OF OPIATES, COCAINE AND CANNABINOIDS IN HAIR [J].
JURADO, C ;
GIMENEZ, MP ;
MENENDEZ, M ;
REPETTO, M .
FORENSIC SCIENCE INTERNATIONAL, 1995, 70 (1-3) :165-174
[6]  
Miller J. C., 1993, STAT ANAL CHEM
[7]   DRUG DETECTION IN HAIR BY CHROMATOGRAPHIC PROCEDURES [J].
MOELLER, MR .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 580 (1-2) :125-134
[8]   SIMULTANEOUS DETERMINATION OF DRUGS OF ABUSE (OPIATES, COCAINE AND AMPHETAMINE) IN HUMAN HAIR BY GC/MS AND ITS APPLICATION TO A METHADONE TREATMENT PROGRAM [J].
MOELLER, MR ;
FEY, P ;
WENNIG, R .
FORENSIC SCIENCE INTERNATIONAL, 1993, 63 (1-3) :185-206
[9]   Application of hair analysis to document coercive heroin administration to a child [J].
Rossi, SS ;
Offidani, C ;
Chiarotti, M .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1998, 22 (01) :75-77
[10]  
TABERNERO MJ, 1995, THESIS U SANTIAGO CO