Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants

被引：105

作者：

Sohlenkamp, C

Shelden, M

Howitt, S

Udvardi, M

机构：

[1] Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany

[2] Australian Natl Univ, Div Biochem & Mol Biol, Canberra, ACT 0200, Australia

来源：

FEBS LETTERS | 2000年 / 467卷 / 2-3期

基金：

澳大利亚研究理事会;

关键词：

ammonium transporter; methylammonium; nitrogen regulation; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(00)01153-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and characterized the first member of a novel family of ammonium transporters in plants: AtAMT2 from Arabidopsis thaliana. AtAMT2 is more closely related to bacterial ammonium transporters than to plant transporters of the AMT1 family. The protein was expressed and functionally characterized in yeast. AtAMT2 transported ammonium in an energy-dependent manner. In contrast to transporters of the AMT1 family, however, AtAMT2 did not transport the ammonium analogue, methylammonium. AtAMT2 was expressed more highly in shoots than roots and was subject to nitrogen regulation. (C) 2000 Federation of European Biochemical Societies.

引用

页码：273 / 278

页数：6

共 29 条

[1] THE EFFECT OF AMMONIUM-IONS ON MEMBRANE-POTENTIAL AND ANION FLUX IN ROOTS OF BARLEY AND TOMATO [J].