Detection of Helicobacter pylori DNA in drinking water biofilms:: implications for transmission in early life

被引:55
作者
Bunn, JEG
MacKay, WG
Thomas, JE
Reid, DC
Weaver, LT
机构
[1] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England
[2] MRC Dunn, Nutr Unit, Cambridge, England
[3] Univ Glasgow, Dept Child Hlth, Glasgow G12 8QQ, Lanark, Scotland
[4] Univ Newcastle Upon Tyne, Dept Child Hlth, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[5] Scottish Execut, Environm Grp, Water Serv Unit, Edinburgh, Midlothian, Scotland
关键词
D O I
10.1046/j.1472-765X.2002.01122.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To provide evidence of water quality as a risk factor for acquisition of Helicobacter pylori in early life, and to identify evidence for its presence within pots used to store drinking water. Methods and Results: A prospective cohort study of 65 infants was conducted in the rural village of Keneba, The Gambia. Age of H. pylori colonization was determined and water pot biofilms were tested for H. pylori by sequencing of amplified DNA. Use of supplemental water was a strong risk factor for H. pylori colonization in infants (OR 4.71, 95% CI 1.17-22.5). DNA with 95% homology to the 16S rRNA gene of H. pylori was isolated from biofilms of water pots. Conclusions: Drinking water may be a reservoir for H. pylori in areas of the developing world where water quality is poor. Early introduction of water, particularly if stored in, or collected from contaminated sources, may be associated with an increased rate of H. pylori colonization.
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收藏
页码:450 / 454
页数:5
相关论文
共 29 条
  • [1] The transmission of Helicobacter pylori via exposure to common sources outweighs the person-to-person contact among spouses in developing countries
    Akcan, Y
    Ersan, S
    Alper, M
    Bicik, Z
    Aytug, N
    [J]. AMERICAN JOURNAL OF GASTROENTEROLOGY, 2000, 95 (01) : 317 - 319
  • [2] Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
    Alm, RA
    Ling, LSL
    Moir, DT
    King, BL
    Brown, ED
    Doig, PC
    Smith, DR
    Noonan, B
    Guild, BC
    deJonge, BL
    Carmel, G
    Tummino, PJ
    Caruso, A
    Uria-Nickelsen, M
    Mills, DM
    Ives, C
    Gibson, R
    Merberg, D
    Mills, SD
    Jiang, Q
    Taylor, DE
    Vovis, GF
    Trost, TJ
    [J]. NATURE, 1999, 397 (6715) : 176 - 180
  • [3] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [4] [Anonymous], 1998, WHONUT981
  • [5] Buswell CM, 1998, APPL ENVIRON MICROB, V64, P733
  • [6] BIOFILMS, THE CUSTOMIZED MICRONICHE
    COSTERTON, JW
    LEWANDOWSKI, Z
    DEBEER, D
    CALDWELL, D
    KORBER, D
    JAMES, G
    [J]. JOURNAL OF BACTERIOLOGY, 1994, 176 (08) : 2137 - 2142
  • [7] Helicobacter pylori infection, gastric acid secretion, and infant growth
    Dale, A
    Thomas, JE
    Darboe, MK
    Coward, WA
    Harding, M
    Weaver, LT
    [J]. JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1998, 26 (04) : 393 - 397
  • [8] REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION AMPLIFICATION OF RIBOSOMAL-RNA FOR DETECTION OF HELICOBACTER SPECIES
    ENGSTRAND, L
    NGUYEN, AMH
    GRAHAM, DY
    ELZAATARI, FAK
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (09) : 2295 - 2301
  • [9] Helicobacter in water and waterborne routes of transmission
    Engstrand, L
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2001, 90 : 80S - 84S
  • [10] FORMAN D, 1993, LANCET, V341, P1359