DETECTION OF BEHAVIORAL ALTERATIONS AND LEARNING DEFICITS IN MICE LACKING SYNAPTOPHYSIN

被引:258
作者
Schmitt, U. [1 ]
Tanimoto, N. [3 ]
Seeliger, M. [3 ]
Schaeffel, F. [4 ]
Leube, R. E. [2 ]
机构
[1] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Dept Psychiat & Psychotherapy, D-55101 Mainz, Germany
[2] Rhein Westfal TH Aachen, Inst Mol & Cellular Anat, D-52074 Aachen, Germany
[3] Univ Tubingen, Ctr Ophthalmol, Inst Ophthalm Res, Div Ocular Neurodegenerat, D-72076 Tubingen, Germany
[4] Univ Eye Hosp, Sect Neurobiol Eye, D-72076 Tubingen, Germany
关键词
transgenic mice; synaptophysin; behavior; learning; electroretinogram; optokinetics; SYNAPTIC VESICLE EXOCYTOSIS; ELEVATED PLUS-MAZE; TYROSINE PHOSPHORYLATION; ENRICHED ENVIRONMENT; RESTRAINT STRESS; RAT HIPPOCAMPUS; TRANSGENIC MICE; MOUSE MODEL; OPEN-FIELD; EXPRESSION;
D O I
10.1016/j.neuroscience.2009.04.046
中图分类号
Q189 [神经科学];
学科分类号
071006 [神经生物学];
摘要
The integral membrane protein synaptophysin is one of the most abundant polypeptide components of synaptic vesicles. It is not essential for neurotransmission despite its abundance but is believed to modulate the efficiency of the synaptic vesicle cycle. Detailed behavioral analyses were therefore performed on synaptophysin knockout mice to test whether synaptophysin affects higher brain functions. We find that these animals are more exploratory than their wild type counterparts examining novel objects more closely and intensely in an enriched open field arena. We also detect impairments in learning and memory, most notably reduced object novelty recognition and reduced spatial learning. These deficits are unlikely caused by impaired vision, since all electroretinographic parameters measured were indistinguishable from those in wild type controls although an inverse optomotor reaction was observed. Taken together, our observations demonstrate functional consequences of synaptophysin depletion in a living organism. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:234 / 243
页数:10
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