Perineural Dexmedetomidine Attenuates Inflammation in Rat Sciatic Nerve via the NF-κB Pathway

被引：85

作者：

Huang, Yan ^{[1
]}

Lu, Yi ^{[1
]}

Zhang, Lei ^{[1
]}

Yan, Jia ^{[1
]}

Jiang, Jue ^{[1
]}

Jiang, Hong ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Anesthesiol, Sch Med, Shanghai Peoples Hosp 9, Shanghai 200011, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2014年 / 15卷 / 03期

基金：

中国国家自然科学基金;

关键词：

dexmedetomidine; NF-B; IL-6; TNF-; nerve block; PYRROLIDINE DITHIOCARBAMATE; BLOCK; ROPIVACAINE; BUPIVACAINE; ANESTHESIA; ANALGESIA; PROPOFOL; DURATION; INCREASE; PATIENT;

D O I：

10.3390/ijms15034049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Recent studies have shown that dexmedetomidine exerts an anti-inflammatory effect by reducing serum levels of inflammatory factors, however, the up-stream mechanism is still unknown. The transcription factor NF-B enters the nucleus and promotes the transcription of its target genes, including those encoding the pro-inflammatory cytokines IL-6 and TNF-. In this study, we established a rat model that simulates a clinical surgical procedure to investigate the anti-inflammatory effect of perineural administration of dexmedetomidine and the underlying mechanism. Dexmedetomidine reduced the sciatic nerve levels of IL-6 and TNF- at both the mRNA and protein level. Dexmedetomidine also inhibited the translocation of activated NF-B to the nucleus and the binding activity of NF-B. The anti-inflammatory effect is confirmed to be dose-dependent. Finally, pyrrolidine dithiocarbamate also reduced the levels of IL-6 and TNF- and the activation of NF-B. In conclusion, dexmedetomidine inhibited the nuclear translocation and binding activity of activated NF-B, thus reducing inflammatory cytokines.

引用

页码：4049 / 4059

页数：11

共 22 条

[1]

Perineural administration of dexmedetomidine in combination with bupivacaine enhances sensory and motor blockade in sciatic nerve block without inducing neurotoxicity in rat [J].