Multiple site mutagenesis with high targeting efficiency in one cloning step

被引：21

作者：

Kim, YG ^{[1
]}

Maas, S ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

BIOTECHNIQUES | 2000年 / 28卷 / 02期

关键词：

D O I：

10.2144/00282bm01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：196 / 198

页数：3

共 9 条

[1] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[2]

Ishii TM, 1998, METHOD ENZYMOL, V293, P53

[3] An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes [J].

Kirsch, RD ;

Joly, E .

NUCLEIC ACIDS RESEARCH, 1998, 26 (07) :1848-1850

[4] RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION [J].

KUNKEL, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :488-492

[5]

NELSON M, 1992, METHOD ENZYMOL, V216, P279

[6]

Papworth C., 1996, Strategies, V9, P3, DOI DOI 10.1080/08924562.1996.11000299

[7]

Sambrook J., 2002, MOL CLONING LAB MANU

[8]

SCOTT B, 1994, STRATEGIES, V7, P62

[9] SITE-DIRECTED MUTAGENESIS OF DOUBLE-STRANDED DNA BY THE POLYMERASE CHAIN-REACTION [J].

WEINER, MP ;

COSTA, GL ;

SCHOETTLIN, W ;

CLINE, J ;

MATHUR, E ;

BAUER, JC .

GENE, 1994, 151 (1-2) :119-123

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