Characterization of nine polymorphic microsatellite loci in the fungus Botrytis cinerea (Ascomycota)

被引：82

作者：

Fournier, E

Giraud, T

Loiseau, A

Vautrin, D

Estoup, A

Solignac, M

Cornuet, JM

Brygoo, Y

机构：

[1] INRA, PMDV, F-78026 Versailles, France

[2] Univ Paris 11, ESE, F-91405 Orsay, France

[3] CBGP, F-34988 Montferrier Sur Lez, France

[4] CNRS, F-91198 Gif Sur Yvette, France

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期

关键词：

Ascomycetes; BLAST; Botryotinia fuckeliana; fungus; grey mold; species complex;

D O I：

10.1046/j.1471-8286.2002.00207.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine microsatellite markers were characterized in the fungus Botrytis cinerea. Genomic DNA sequences from the partial sequencing of 12 000 bacterial artificial chromosome (BAC) clones, were screened by BLAST for various microsatellite motives, and primer pairs were designed. Cross-amplification and polymorphism were assessed on 49 isolates from B. cinerea and two related species, collected from natural populations on several plants and locations.

引用

页码：253 / 255

页数：3

共 6 条

[1] BOTY, A LONG-TERMINAL-REPEAT RETROELEMENT IN THE PHYTOPATHOGENIC FUNGUS BOTRYTIS-CINEREA [J].