Characterization of nine polymorphic microsatellite loci in the fungus Botrytis cinerea (Ascomycota)

被引:82
作者
Fournier, E
Giraud, T
Loiseau, A
Vautrin, D
Estoup, A
Solignac, M
Cornuet, JM
Brygoo, Y
机构
[1] INRA, PMDV, F-78026 Versailles, France
[2] Univ Paris 11, ESE, F-91405 Orsay, France
[3] CBGP, F-34988 Montferrier Sur Lez, France
[4] CNRS, F-91198 Gif Sur Yvette, France
来源
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期
关键词
Ascomycetes; BLAST; Botryotinia fuckeliana; fungus; grey mold; species complex;
D O I
10.1046/j.1471-8286.2002.00207.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nine microsatellite markers were characterized in the fungus Botrytis cinerea. Genomic DNA sequences from the partial sequencing of 12 000 bacterial artificial chromosome (BAC) clones, were screened by BLAST for various microsatellite motives, and primer pairs were designed. Cross-amplification and polymorphism were assessed on 49 isolates from B. cinerea and two related species, collected from natural populations on several plants and locations.
引用
收藏
页码:253 / 255
页数:3
相关论文
共 6 条
[1]   BOTY, A LONG-TERMINAL-REPEAT RETROELEMENT IN THE PHYTOPATHOGENIC FUNGUS BOTRYTIS-CINEREA [J].
DIOLEZ, A ;
MARCHES, F ;
FORTINI, D ;
BRYGOO, Y .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (01) :103-108
[2]   Two sibling species of the Botrytis cinerea complex, transposa and vacuma, are found in sympatry on numerous host plants [J].
Giraud, T ;
Fortini, D ;
Levis, C ;
Lamarque, C ;
Leroux, P ;
LoBuglio, K ;
Brygoo, Y .
PHYTOPATHOLOGY, 1999, 89 (10) :967-973
[3]   RFLP markers show genetic recombination in Botryotinia fuckeliana (Botrytis cinerea) and transposable elements reveal two sympatric species [J].
Giraud, T ;
Fortini, D ;
Levis, C ;
Leroux, P ;
Brygoo, Y .
MOLECULAR BIOLOGY AND EVOLUTION, 1997, 14 (11) :1177-1185
[4]  
MOHAVEDI S, 1990, PHYSIOL MOL PLANT P, V36, P303
[5]   A SIMPLE AND EFFICIENT PROTOCOL FOR ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM FILAMENTOUS FUNGI, FRUIT BODIES, AND INFECTED-PLANT TISSUES [J].
MOLLER, EM ;
BAHNWEG, G ;
SANDERMANN, H ;
GEIGER, HH .
NUCLEIC ACIDS RESEARCH, 1992, 20 (22) :6115-6116
[6]  
Nei M., 1987, Science, Philosophy and Human Behavior in the Soviet Union