Biotransformation of p-xylene and 2,6-dimethylnaphthalene by xylene monooxygenase cloned from a Sphingomonas isolate

被引：15

作者：

Bramucci, M ^{[1
]}

Singh, M ^{[1
]}

Nagarajan, V ^{[1
]}

机构：

[1] DuPont Co Inc, Cent Res & Dev, Wilmington, DE 19880 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 59卷 / 06期

关键词：

D O I：

10.1007/s00253-002-1068-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sphingomonas strain ASU1 was isolated from an industrial wastewater bioreactor and grew on 2,6-dimethylnaphthalene (2,6-DMN) as the sole carbon/energy source. The genes for a xylene monooxygenase were cloned from strain ASU1. Expression of the ASU1 xylene monooxygenase was compared to expression of the pWWO xylene monooxygenase in Escherichia coli. Both monooxygenases transformed p-xylene and 2,6-DMN by initially hydroxylating one methyl group. In addition, the ASU1 monooxygenase also hydroxylated the second methyl group on p-xylene and 2,6-DMN whereas the pWWO monooxygenase hydroxylated the second methyl group only on p-xylene. Endogenous E. coli enzymes contributed to further oxidation of the resulting aromatic alcohols to form aromatic carboxylates.

引用

页码：679 / 684

页数：6

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