Second-harmonic-generation microscope with a microlens array scanner

被引：68

作者：

Kobayashi, M

Fujita, K

Kaneko, T

Takamatsu, T

Nakamura, O

Kawata, S

机构：

[1] Osaka Univ, Dept Appl Phys, Handai Frontier Res Ctr, Suita, Osaka 565, Japan

[2] Kyoto Prefectural Univ Med, Dept Pathol & Cell Regulat, Kamigyo Ku, Kyoto, Japan

来源：

OPTICS LETTERS | 2002年 / 27卷 / 15期

关键词：

D O I：

10.1364/OL.27.001324

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We propose a multifocus scanning technique in second-harmonic-generation (SHG) microscopy in which the SHG detection efficiency and the image acquisition rate are improved by several tens of times compared with typical single-focus scanning techniques. Because of a microlens array scanner, the laser beam of a mode-locked Ti:sapphire laser is split into similar to100 beamlets, and the same number of foci are formed in the specimen. Parallel scanning of the foci enables us to observe real-time images of the specimen. The imaging properties and the usefulness of the microscope are demonstrated by SHG images of living HeLa cells and rat cardiac myocytes. (C) 2002 Optical Society of America.

引用

页码：1324 / 1326

页数：3

共 11 条

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