TtcA a new tRNA-thioltransferase with an Fe-S cluster

被引：53

作者：

Bouvier, Denis ^{[1
,2
,3
]}

Labessan, Natty ^{[1
,2
,3
]}

Clemancey, Martin ^{[1
,2
,3
]}

Latour, Jean-Marc ^{[1
,2
,3
]}

Ravanat, Jean-Luc ^{[4
,5
]}

Fontecave, Marc ^{[1
,2
,3
,6
]}

Atta, Mohamed ^{[1
,2
,3
]}

机构：

[1] Univ Grenoble Alpes, iRTSV LCBM, UMR5249, F-38000 Grenoble, France

[2] CNRS, iRTSV LCBM, UMR5249, F-38000 Grenoble, France

[3] CEA Grenoble, iRTSV LCBM, UMR5249, F-38000 Grenoble, France

[4] Univ Grenoble Alpes, INAC, SCIB, F-38000 Grenoble, France

[5] CEA Grenoble, iNAC, SCIB, F-38054 Grenoble, France

[6] UPMC, CNRS, UMR Coll France 8229, Lab Chim Proc Biol, Paris, France

来源：

NUCLEIC ACIDS RESEARCH | 2014年 / 42卷 / 12期

关键词：

ENTERICA SEROVAR TYPHIMURIUM; PERFORMANCE LIQUID-CHROMATOGRAPHY; THIOLATED NUCLEOSIDES PRESENT; CYSTEINE DESULFURASE ISCS; IRON-SULFUR PROTEIN; ESCHERICHIA-COLI; 4-THIOURIDINE BIOSYNTHESIS; ENZYME; THIONUCLEOSIDES; IDENTIFICATION;

D O I：

10.1093/nar/gku508

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

TtcA catalyzes the post-transcriptional thiolation of cytosine 32 in some tRNAs. The enzyme from Escherichia coli was homologously overexpressed in E. coli. The purified enzyme is a dimer containing an iron-sulfur cluster and displays activity in in vitro assays. The type and properties of the cluster were investigated using a combination of UV-visible absorption, EPR and Mossbauer spectroscopy, as well as by site-directed mutagenesis. These studies demonstrated that the TtcA enzyme contains a redox-active and oxygen-sensitive [4Fe-4S] cluster, chelated by only three cysteine residues and absolutely essential for activity. TtcA is unique tRNA-thiolating enzyme using an iron-sulfur cluster for catalyzing a non-redox reaction.

引用

页码：7960 / 7970

页数：11

共 30 条

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