Influence of different anticoagulants on platelet aggregation in whole blood; A comparison between citrate, low molecular mass heparin and hirudin

被引:76
作者
Wallen, NH [1 ]
Ladjevardi, M [1 ]
Albert, J [1 ]
Broijersen, A [1 ]
机构
[1] DANDERYD HOSP,DEPT ANESTHESIOL,STOCKHOLM,SWEDEN
关键词
platelet aggregation; citrate; hirudin; low molecular mass heparin; impedance aggregometry; aspirin;
D O I
10.1016/S0049-3848(97)00114-X
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Anticoagulants used for platelet function studies in vitro may affect platelet responsiveness. Tn the present study we compared the influence of three different anticoagulants, sodium citrate, low molecular mass heparin, and recombinant hirudin, on platelet aggregation in whole blood in vitro, using impedance aggregometry. ADP and collagen induced aggregation was significantly lower in citrated blood compared to hirudin treated blood, reflecting the importance of exctracellular calcium for platelet function. Inhibition of platelet aggregation by aspirin, was more pronounced in citrated blood compared to hirudin treated blood, in agreement with the concept of artifactually enhanced thromboxane generation in media containing low extracellular calcium levels. In blood anticoagulated with low molecular mass heparin, platelet aggregation to collagen tended to be enhanced as compared to hirudin treated blood, whereas platelet responses to ADP at a high concentration were slightly reduced. Of the anticoagulants investigated, the selective thrombin inhibitor hirudin is the most suitable anticoagulant for studies of platelet aggregation in vitro in whole blood. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:151 / 157
页数:7
相关论文
共 17 条
[1]   COMPARISON OF THE PLATELET PRO-AGGREGATORY EFFECT OF CONVENTIONAL UNFRACTIONATED HEPARINS AND A LOW-MOLECULAR-WEIGHT HEPARIN FRACTION (CY-222) [J].
BARRADAS, MA ;
MIKHAILIDIS, DP ;
EPEMOLU, O ;
JEREMY, JY ;
FONSECA, V ;
DANDONA, P .
BRITISH JOURNAL OF HAEMATOLOGY, 1987, 67 (04) :451-457
[2]   ON THE ACCURATE DETERMINATION OF SEROTONIN IN HUMAN PLASMA [J].
BECK, O ;
WALLEN, NH ;
BROIJERSEN, A ;
LARSSON, PT ;
HJEMDAHL, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 196 (01) :260-266
[3]   PLATELET ACTIVATION [J].
BLOCKMANS, D ;
DECKMYN, H ;
VERMYLEN, J .
BLOOD REVIEWS, 1995, 9 (03) :143-156
[4]   HEPARIN AND LOW-MOLECULAR-WEIGHT HEPARIN BUT NOT HIRUDIN STIMULATE PLATELET-AGGREGATION IN WHOLE-BLOOD FROM ACETYLSALICYLIC-ACID TREATED HEALTHY-VOLUNTEERS [J].
CHEN, JH ;
KARLBERG, KE ;
SYLVEN, C .
THROMBOSIS RESEARCH, 1991, 63 (03) :319-329
[5]   ASPIRIN DOES NOT AFFECT THE NOW CYTOMETRIC DETECTION OF FIBRINOGEN BINDING TO, OR RELEASE OF ALPHA-GRANULES OR LYSOSOMES FROM, HUMAN PLATELETS [J].
CHRONOS, NAF ;
WILSON, DJ ;
JANES, SL ;
HUTTON, RA ;
BULLER, NP ;
GOODALL, AH .
CLINICAL SCIENCE, 1994, 87 (05) :575-580
[6]  
COLMAN RW, 1991, P SOC EXP BIOL MED, V197, P242
[7]   ANTI-COAGULANT ACTIVITIES AND EFFECTS ON PLATELETS OF A HEPARIN FRAGMENT WITH HIGH-AFFINITY FOR ANTI-THROMBIN [J].
HOLMER, E ;
LINDAHL, U ;
BACKSTROM, G ;
THUNBERG, L ;
SANDBERG, H ;
SODERSTROM, G ;
ANDERSSON, LO .
THROMBOSIS RESEARCH, 1980, 18 (06) :861-869
[8]  
MARKWARDT F, 1991, THROMB HAEMOSTASIS, V66, P141
[9]   Flow cytometry: A clinical test of platelet function [J].
Michelson, AD .
BLOOD, 1996, 87 (12) :4925-4936
[10]  
PACKHAM MA, 1989, THROMB HAEMOSTASIS, V62, P968