Examination of actin polymerization and viscosity induced by cations and ionic strength when cross-linked by α-actinin

被引：5

作者：

Goldmann, WH ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Med,Renal Unit, Charlestown, MA 02129 USA

来源：

CELL BIOLOGY INTERNATIONAL | 2002年 / 26卷 / 06期

关键词：

alpha-actinin; actin polymerization; viscosity; cations; ionic strength;

D O I：

10.1006/cbir.2002.0898

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Increasing potassium chloride concentration from 0 to 100 mM and magnesium chloride from 0 to 2 mM show a parallel rate increase in polymerizing actin, whereas increasing calcium chloride concentration from 0 to 0.2 mM decreases the rate of polymerizing actin. The presence of alpha-actinin has little influence on the polymerization kinetics of actin under these conditions. Viscometric measurements indicate that the presence of various mono- and divalent cations, ionic strength, and alpha-actinin in combination are responsible for changes in the mechanical properties of solutions containing actin. The actin filament dynamic behavior is drastically reduced under these conditions as confirmed by quasi-elastic light scattering. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：541 / 546

页数：6

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