Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporter

被引：18

作者：

Griffin, JB

Stanley, JS

Zempleni, J

机构：

[1] Univ Nebraska, Dept Nutrit Sci & Dietet, Lincoln, NE 68583 USA

[2] Arkansas Childrens Hosp, Res Inst, Little Rock, AR 72202 USA

来源：

INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH | 2002年 / 72卷 / 04期

关键词：

biotin; human; lipoic acid; pantothenic acid; polyclonal antibody; transport;

D O I：

10.1024/0300-9831.72.4.195

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

In mammalian cells, biotin is covalently attached to carboxylases and histones and is required for cell proliferation and function. Cellular uptake of biotin (as well as pantothenic acid and lipoic acid) is mediated by the sodium-dependent multivitamin transporter, SMVT. Studies of cellular biotin homeostasis have been hampered by the lack of an antibody to SMVT. Here, we describe the synthesis of a rabbit polyclonal antibody to human SMVT. Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT.

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页码：195 / 198

页数：4

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