Ageing is reversed, and metabolism is reset to young levels in recovering dauer larvae of C-elegans

被引:65
作者
Houthoofd, K [1 ]
Braeckman, BP [1 ]
Lenaerts, I [1 ]
Brys, K [1 ]
De Vreese, A [1 ]
Van Eygen, S [1 ]
Vanfleteren, JR [1 ]
机构
[1] Univ Ghent, Dept Biol, B-9000 Ghent, Belgium
基金
美国国家卫生研究院;
关键词
ageing; Caenorhabditis elegans; dauer; biochemistry; longevity; life span; metabolism; rejuvenation;
D O I
10.1016/S0531-5565(02)00063-3
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
The nematode Caenorhabditis elegans responds to unfavourable environmental conditions by arresting development and entering diapause as a dauer larva. Dauers can survive several times the normal life span and the duration of the dauer state has no effect on postdauer life span. This led to the suggestion that dauers are non-ageing, and that dauers eventually perish as the consequence of depletion of stored nutrients. We have investigated physiological changes associated with long-term diapause survival, and found that dauer larvae slowly develop senescence-like symptoms, including decrease of metabolic capacity, aconitase enzyme activity, and ATP stores, and increase of lipofuscin- and oxidised flavin-specific fluorescence. However, these changes are reversed when the dauers recover. Thus senescence can occur before attainment of reproductive maturity, and furthermore, is reversible. Other life processes, including respiration rate and heat output, remain unaltered over four weeks of diapause at 24 degreesC. Possible determinants of the enhanced life maintenance include increased resistance to oxidative stress provided by enhanced superoxide dismutase and catalase activities, and a shift to a highly reducing redox status. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:1015 / 1021
页数:7
相关论文
共 31 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   MUTANTS OF CAENORHABDITIS-ELEGANS THAT FORM DAUER-LIKE LARVAE [J].
ALBERT, PS ;
RIDDLE, DL .
DEVELOPMENTAL BIOLOGY, 1988, 126 (02) :270-293
[4]   Assaying metabolic activity in ageing Caenorhabditis elegans [J].
Braeckman, BP ;
Houthoofd, K ;
De Vreese, A ;
Vanfleteren, JR .
MECHANISMS OF AGEING AND DEVELOPMENT, 2002, 123 (2-3) :105-119
[5]   Apparent uncoupling of energy production and consumption in long-lived Clk mutants of Caenorhabditis elegans [J].
Braeckman, BP ;
Houthoofd, K ;
De Vreese, A ;
Vanfleteren, JR .
CURRENT BIOLOGY, 1999, 9 (09) :493-496
[6]   A NEW TECHNIQUE FOR HIGHLY SENSITIVE DETECTION OF SUPEROXIDE-DISMUTASE ACTIVITY BY CHEMILUMINESCENCE [J].
CORBISIER, P ;
HOUBION, A ;
REMACLE, J .
ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) :240-247
[7]   Selectivity of protein oxidative damage during aging in Drosophila melanogaster [J].
Das, N ;
Levine, RL ;
Orr, WC ;
Sohal, RS .
BIOCHEMICAL JOURNAL, 2001, 360 (360) :209-216
[8]   TOTAL LUMINESCENCE SPECTROSCOPY OF FLUORESCENCE CHANGES DURING AGING IN CAENORHABDITIS-ELEGANS [J].
DAVIS, BO ;
ANDERSON, GL ;
DUSENBERY, DB .
BIOCHEMISTRY, 1982, 21 (17) :4089-4095
[9]   PRODUCTION OF AGE-SYNCHRONOUS MASS-CULTURES OF CAENORHABDITIS-ELEGANS [J].
FABIAN, TJ ;
JOHNSON, TE .
JOURNALS OF GERONTOLOGY, 1994, 49 (04) :B145-B156
[10]   Oxidative biochemical markers; clues to understanding aging in long-lived species [J].
Floyd, RA ;
West, M ;
Hensley, K .
EXPERIMENTAL GERONTOLOGY, 2001, 36 (4-6) :619-640