Some aspects of the venom proteome of the Colubridae snake Philodryas olfersii revealed from a Duvernoy's (venom) gland transcriptome

被引：90

作者：

Ching, Ana T. C.

Rocha, Marisa M. T.

Leme, Adriana F. Paes

Pimenta, Daniel C.

de Fatima D Furtado, Maria

Serrano, Solange M. T.

Ho, Paulo L.

Junqueira-de-Azevedo, Inacio L. M.

机构：

[1] Ist Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo, Brazil

[2] Univ Sao Paulo, BR-05508900 Sao Paulo, Brazil

[3] Inst Butantan, Lab Herpetol, BR-05503900 Sao Paulo, Brazil

[4] Inst Butantan, Lab Especial Toxinol Aplicada, CAT, CEPID, BR-05503900 Sao Paulo, Brazil

来源：

FEBS LETTERS | 2006年 / 580卷 / 18期

基金：

巴西圣保罗研究基金会;

关键词：

snake venom; transcriptome; molecular evolution; natriuretic peptide; 2D electrophoresis; Colubridae; NATRIURETIC PEPTIDE; GREEN SNAKE; SECRETION; EVOLUTION; ELAPIDAE; PROTEINS; CLONING; ORIGIN; WORLD;

D O I：

10.1016/j.febslet.2006.07.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the putative toxins of Philodryas olfersii (Colubridae), a representative of a family of snakes neglected in venom studies despite their growing medical importance. Transcriptomic data of the venom gland complemented by proteomic analysis of the gland secretion revealed the presence of major toxin classes from the Viperidae family, including serine proteases, metalloproteases, C-type lectins, Crisps, and a C-type natriuretic peptide (CNP). Interestingly, the phylogenetic analysis of the CNP precursor showed it as a linker between two related precursors found in Viperidae and Elapidae snakes. We suggest that these precursors constitute a monophyletic group derived from the vertebrate CNPs. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4417 / 4422

页数：6

共 27 条

[1] ISOLATION AND CHARACTERIZATION OF 5 FIBRIN(OGEN)OLYTIC ENZYMES FROM THE VENOM OF PHILODRYAS-OLFERSII (GREEN SNAKE) [J].