DNA sequence recognition by hybridization to short oligomers: Experimental verification of the method on the E-coli genome

被引：15

作者：

Milosavljevic, A ^{[1
]}

Savkovic, S ^{[1
]}

Crkvenjakov, R ^{[1
]}

Salbego, D ^{[1
]}

Serrato, H ^{[1
]}

Kreuzer, H ^{[1
]}

Gemmell, A ^{[1
]}

Batus, S ^{[1
]}

Grujic, D ^{[1
]}

Carnahan, S ^{[1
]}

Paunesku, T ^{[1
]}

Tepavcevic, J ^{[1
]}

机构：

[1] ARGONNE NATL LAB,CTR MECHANIST BIOL & BIOTECHNOL,ARGONNE,IL 60439

来源：

GENOMICS | 1996年 / 37卷 / 01期

关键词：

D O I：

10.1006/geno.1996.0523

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A newly developed method for sequence recognition by hybridization to short oligomers is verified for the first time in genome-scale experiments. The experiments involved hybridization of 15,328 randomly selected 2-kb genomic clones of Escherichia coli with 997 short oligomer probes to detect complementary oligomers within the clones. Lists of oligomers detected within individual clones were compiled into a database. The database was then searched using known E. coli sequences as queries, The goal was to recognize the clones that are identical or similar to the query sequences. A total of 76 putative recognitions were tested in two separate but complementary recognition experiments. The results indicate high specificity of recognition. Current and prospective applications of this novel method are discussed. (C) 1996 Academic Press, Inc.

引用

页码：77 / 86

页数：10

共 21 条

[1]

[Anonymous], [No title captured], DOI DOI 10.1007/3-540-58338-6_

[2]

A MEASURE OF THE SIMILARITY OF SETS OF SEQUENCES NOT REQUIRING SEQUENCE ALIGNMENT [J].

BLAISDELL, BE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (14) :5155-5159

[3]

DNA-SEQUENCE DETERMINATION BY HYBRIDIZATION - A STRATEGY FOR EFFICIENT LARGE-SCALE SEQUENCING [J].

DRMANAC, R ;

DRMANAC, S ;

STREZOSKA, Z ;

PAUNESKU, T ;

LABAT, I ;

ZEREMSKI, M ;

SNODDY, J ;

FUNKHOUSER, WK ;

KOOP, B ;

HOOD, L ;

CRKVENJAKOV, R .

SCIENCE, 1993, 260 (5114) :1649-1653

[4]

DRMANAC R, 1991, ELECTROPHORESES SUPE, P60

[5]

DRMANAC R, 1994, AUTOMATED DNA SEQUEN, P29

[6]

Drmanac RT, 1993, US Patent, Patent No. [5,202,231, 5202231]

[7]

DRMANAC S, 1994, BIOTECHNIQUES, V7, P328

[8]

MULTIPLEXED BIOCHEMICAL ASSAYS WITH BIOLOGICAL CHIPS [J].

FODOR, SPA ;

RAVA, RP ;

HUANG, XHC ;

PEASE, AC ;

HOLMES, CP ;

ADAMS, CL .

NATURE, 1993, 364 (6437) :555-556

[9]

Hide W, 1994, J Comput Biol, V1, P199, DOI 10.1089/cmb.1994.1.199

[10]

HYBRIDIZATION ANALYSES OF ARRAYED CDNA LIBRARIES [J].

LENNON, GG ;

LEHRACH, H .

TRENDS IN GENETICS, 1991, 7 (10) :314-317

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