Signaling aptamers for monitoring enzymatic activity and for inhibitor screening

被引：70

作者：

Nutiu, R

Yu, JMY

Li, YF

机构：

[1] McMaster Univ, Dept Biochem, Hamilton, ON L8N 3Z5, Canada

[2] McMaster Univ, Dept Chem, Hamilton, ON L8N 3Z5, Canada

来源：

CHEMBIOCHEM | 2004年 / 5卷 / 08期

关键词：

aptamers; DNA; enzyme inhibitors; fluorescence; small-molecule screening;

D O I：

10.1002/cbic.200400026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Real-time action: The conversion of adenosine 5′-monophosphate (AMP) into adenosine by alkaline phosphatase was monitored in real time by exploiting a signaling DNA aptamer as a reporter. The aptamer has a higher affinity for adenosine than for AMP and releases readily distinguishable fluorescence signals upon complexation with AMP and after conversion of AMP into adenosine (see figure).

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页码：1139 / 1144

页数：6

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