Detection of glutamate in optically trapped single nerve terminals by Raman spectroscopy

被引:43
作者
Ajito, K [1 ]
Han, CX [1 ]
Torimitsu, K [1 ]
机构
[1] NTT Corp, Basic Res Labs, JST, SORST, Atsugi, Kanagawa 2430198, Japan
关键词
D O I
10.1021/ac049969m
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Glutamate is the major excitatory neurotransmitter and is of particular interest in light of current models of memory and learning. The paper describes the first in situ detection of glutamate in single nerve terminals (synaptosomes), which is achieved by using laser trapping Raman spectroscopy. The near-infrared laser light captures a single synaptosome obtained from a Wister rat brain. The release of glutamate in a single laser-trapped synaptosome was detected by subtracting the Raman spectrum before depolarization from that after depolarization with the addition of the K+-channel blocker, 4-aminopyridine. The result indicated that the single synaptosome released similar to3 amol of glutamate and that the release rate depended on the 4-aminopyridine concentration.
引用
收藏
页码:2506 / 2510
页数:5
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