Mesoporous ZnS Nanorattles: Programmed Size Selected Access to Encapsulated Enzymes

被引：40

作者：

Van Gough, Dara ^{[1
,2
,3
]}

Wolosiuk, Alejandro ^{[4
,5
]}

Braun, Paul V. ^{[1
,2
,3
]}

机构：

[1] Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA

[2] Univ Illinois, Beckman Inst, Urbana, IL 61801 USA

[3] Univ Illinois, Frederick Seitz Mat Res Lab, Urbana, IL 61801 USA

[4] CAC, CNEA, Buenos Aires, DF, Argentina

[5] Univ Buenos Aires, FCEN, DQIAyQF, Buenos Aires, DF, Argentina

来源：

NANO LETTERS | 2009年 / 9卷 / 05期

基金：

美国国家科学基金会;

关键词：

SILICA PARTICLES; HOLLOW SPHERES; PEROXIDASE; MECHANISM; RELEASE; PAPAIN; SHELLS; STABILITY; OXIDASE;

D O I：

10.1021/nl900264n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A size selective nanorattle was formed by encapsulating soybean peroxidase (SBP) within a ZnS mesoporous hollow sphere. Once encapsulated within the mesoporous hollow sphere, the SBP remained active against molecules smaller than the 3 nm diameter of the mesopores in the shell wall, while molecules larger than the mesopores, which could not pass into the hollow sphere, did not interact with the SBP. Specifically, encapsulated SBP catalyzed the oxidation of Amplex Ultra-Red, a small fluorogen, in the presence of hydrogen peroxide, encapsulated SBP was deactivated by sodium azide, and no reaction was observed between encapsulated SBP and a greater than 3 nm diameter protease.

引用

页码：1994 / 1998

页数：5

共 36 条

[1] PEROXIDASE-CATALYZED POLYMERIZATION AND DEPOLYMERIZATION OF COAL IN ORGANIC-SOLVENTS [J].