A comparison of screening methods for antioxidant activity in seaweeds

被引：178

作者：

Matsukawa, R

Dubinsky, Z

Kishimoto, E

Masaki, K

Masuda, Y

Takeuchi, T

Chihara, M

Yamamoto, Y

Niki, E

Karube, I

机构：

[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,MEGURO KU,TOKYO 153,JAPAN

[2] BAR ILAN UNIV,DEPT LIFE SCI,IL-52900 RAMAT GAN,ISRAEL

[3] SCI UNIV TOKYO,FAC ENGN,SHINJUKU KU,TOKYO 162,JAPAN

[4] TOYO SUSAN KAISHA LTD,MINATO KU,TOKYO 108,JAPAN

[5] HIROSHIMA CITY UNIV,FAC INFORMAT SCI,ASAMINAMI KU,HIROSHIMA 73131,JAPAN

[6] JAPANESE RED CROSS COLL NURSING,SHIBUYA KU,TOKYO 150,JAPAN

来源：

JOURNAL OF APPLIED PHYCOLOGY | 1997年 / 9卷 / 01期

关键词：

antioxidant activity; screening; lipoxygenase inhibition; radical scavenging activity; seaweed;

D O I：

10.1023/A:1007935218120

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by alpha, alpha-diphenyl-beta-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.

引用

页码：29 / 35

页数：7

共 23 条

[1] LINOLEATE OXIDATION INDUCED BY LIPOXYGENASE AND HEME PROTEINS - A DIRECT SPECTROPHOTOMETRIC ASSAY [J].