Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity

被引：51

作者：

Higuchi, Akon ^{[1
,2
,3
,4
]}

Kao, Shih-Hsuan ^{[1
]}

Ling, Qing-Dong ^{[5
,6
]}

Chen, Yen-Ming ^{[1
]}

Li, Hsing-Fen ^{[1
]}

Alarfaj, Abdullah A. ^{[4
]}

Munusamy, Murugan A. ^{[4
]}

Murugan, Kadarkarai ^{[7
]}

Chang, Shih-Chang ^{[8
]}

Lee, Hsin-Chung ^{[8
,9
]}

Hsu, Shih-Tien ^{[10
]}

Kumar, S. Suresh ^{[11
]}

Umezawa, Akihiro ^{[2
]}

机构：

[1] Natl Cent Univ, Dept Chem & Mat Engn, Taoyuan 32001, Taiwan

[2] Natl Res Inst Child Hlth & Dev, Dept Reprod, Setagaya Ku, Tokyo 1578535, Japan

[3] RIKEN, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

[4] King Saud Univ, Dept Bot & Microbiol, Coll Sci, Riyadh 11451, Saudi Arabia

[5] Cathay Gen Hosp, Cathay Med Res Inst, Taipei 221, Taiwan

[6] Natl Cent Univ, Grad Inst Syst Biol & Bioinformat, Taoyuan 32001, Taiwan

[7] Bharathiar Univ, Sch Life Sci, Div Entomol, Dept Zool, Coimbatore, Tamil Nadu, India

[8] Cathay Gen Hosp, Dept Surg, Taipei 10693, Taiwan

[9] Natl Cent Univ, Grad Inst Translat & Interdisciplinary Med, Coll Hlth Sci & Technol, Taoyuan 32001, Taiwan

[10] Taiwan Landseed Hosp, Dept Internal Med, Taoyuan 32405, Taiwan

[11] Univ Putra Malaysia, Dept Med Microbiol & Parasitol, Serdang 43400, Slangor, Malaysia

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

SYNTHETIC-POLYMER COATINGS; INSULIN-PRODUCING CELLS; FEEDER-FREE CULTURE; SELF-RENEWAL; DEFINED CONDITIONS; RECOMBINANT VITRONECTIN; EXPANSION; DIFFERENTIATION; DERIVATION; SURFACE;

D O I：

10.1038/srep18136

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The tentative clinical application of human pluripotent stem cells (hPSCs), such as human embryonic stem cells and human induced pluripotent stem cells, is restricted by the possibility of xenogenic contamination resulting from the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. Therefore, we investigated hPSC cultures on biomaterials with different elasticities that were grafted with different nanosegments. We prepared dishes coated with polyvinylalcohol-co-itaconic acid hydrogels grafted with an oligopeptide derived from vitronectin (KGGPQVTRGDVFTMP) with elasticities ranging from 10.3 to 30.4 kPa storage moduli by controlling the crosslinking time. The hPSCs cultured on the stiffest substrates (30.4 kPa) tended to differentiate after five days of culture, whereas the hPSCs cultured on the optimal elastic substrates (25 kPa) maintained their pluripotency for over 20 passages under xeno-free conditions. These results indicate that cell culture matrices with optimal elasticity can maintain the pluripotency of hPSCs in culture.

引用

页数：16

共 56 条

[1]

Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via αVβ5 integrin [J].

Braam, Stefan R. ;

Zeinstra, Laura ;

Litjens, Sandy ;

Ward-van Oostwaard, Dorien ;

van den Brink, Stieneke ;

van Laake, Linda ;

Lebrin, Franck ;

Kats, Peter ;

Hochstenbach, Ron ;

Passier, Robert ;

Sonnenberg, Arnoud ;

Mummery, Christine L. .

STEM CELLS, 2008, 26 (09) :2257-2265

[2]

Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces [J].

Brafman, David A. ;

Chang, Chien W. ;

Fernandez, Antonio ;

Willert, Karl ;

Varghese, Shyni ;

Chien, Shu .

BIOMATERIALS, 2010, 31 (34) :9135-9144

[3]

Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells [J].

Chang, Chien-Wen ;

Hwang, Yongsung ;

Brafman, Dave ;

Hagan, Thomas ;

Phung, Catherine ;

Varghese, Shyni .

BIOMATERIALS, 2013, 34 (04) :912-921

[4]

Chen GK, 2011, NAT METHODS, V8, P424, DOI [10.1038/NMETH.1593, 10.1038/nmeth.1593]

[5]

Thermoresponsive Worms for Expansion and Release of Human Embryonic Stem Cells [J].

Chen, Xiaoli ;

Prowse, Andrew B. J. ;

Jia, Zhongfan ;

Tellier, Helena ;

Munro, Trent P. ;

Gray, Peter P. ;

Monteiro, Michael J. .

BIOMACROMOLECULES, 2014, 15 (03) :844-855

[6]

Soft Substrates Promote Homogeneous Self-Renewal of Embryonic Stem Cells via Downregulating Cell-Matrix Tractions [J].

Chowdhury, Farhan ;

Li, Yanzhen ;

Poh, Yeh-Chuin ;

Yokohama-Tamaki, Tamaki ;

Wang, Ning ;

Tanaka, Tetsuya S. .

PLOS ONE, 2010, 5 (12)

[7]

X-ray photoelectron spectroscopy reference data for identification of the C3N4 phase in carbon-nitrogen films [J].

Dementjev, AP ;

de Graaf, A ;

van de Sanden, MCM ;

Maslakov, KI ;

Naumkin, AV ;

Serov, AA .

[8]

Long-term self-renewal of human pluripotent stem cells on peptide-decorated poly(OEGMA-co-HEMA) brushes under fully defined conditions [J].

Deng, Y. ;

Zhang, X. ;

Zhao, X. ;

Li, Q. ;

Ye, Z. ;

Li, Z. ;

Liu, Y. ;

Zhou, Y. ;

Ma, H. ;

Pan, G. ;

Pei, D. ;

Fang, J. ;

Wei, S. .

ACTA BIOMATERIALIA, 2013, 9 (11) :8840-8850

[9]

Matrix elasticity directs stem cell lineage specification [J].

Engler, Adam J. ;

Sen, Shamik ;

Sweeney, H. Lee ;

Discher, Dennis E. .

CELL, 2006, 126 (04) :677-689

[10]

Fan YJ, 2014, TISSUE ENG PT A, V20, P588, DOI 10.1089/ten.TEA.2013.0219

← 1 2 3 4 5 6 →