Use of LC Fingerprinting to Evaluate the Quality of Erxian Decoction

被引：6

作者：

Hu, Yong Mei ^{[1
]}

Sze, Stephen Cho Wing ^{[1
]}

Zhao, Shou Xun ^{[2
]}

Tong, Yao ^{[1
]}

机构：

[1] Univ Hong Kong, Sch Chinese Med, Pokfulam, Hong Kong, Peoples R China

[2] China Pharmaceut Univ, Dept Phytochem, Nanjing 210009, Peoples R China

来源：

CHROMATOGRAPHIA | 2009年 / 70卷 / 3-4期

关键词：

Column liquid chromatography; Diode-array detection; Erxian decoction; Traditional Chinese medicine; Quality evaluation; Fingerprint; Consistency; PERFORMANCE LIQUID-CHROMATOGRAPHY; CHINESE HERBAL FORMULA; FLAVONOIDS; EXTRACTION; GLYCOSIDES; BERBERINE;

D O I：

10.1365/s10337-009-1218-5

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

High-performance liquid chromatography with diode-array detection has been used to evaluate the quality of Erxian decoction (EXD), a Chinese medicine prescription, by use of a combination of fingerprinting and simultaneous quantification of six marker compounds. The standard chromatographic fingerprint containing forty common peaks was constructed from seven batches of the EXD samples to evaluate batch-to-batch consistency. In addition, six representative bioactive markers were simultaneously quantified with good linearity, precision, repeatability, and accuracy. The fingerprint accompanied by quantification of the marker compounds can be used to evaluate the quality of EXD.

引用

页码：613 / 618

页数：6

共 24 条

[1]

[Anonymous], 2007, CENTRAL S PHARM

[2]

[Anonymous], 2000, CHIN TRADIT PAT MED, V22, P671

[3]

Analysis of berberine and total alkaloid content in Cortex Phellodendri by near infrared spectroscopy (NIRS) compared with high-performance liquid chromatography coupled with ultra-visible spectrometric detection [J].

Chan, Chi-On ;

Chu, Ching-Ching ;

Mok, Daniel Kam-Wah ;

Chau, Foo-Tim .

ANALYTICA CHIMICA ACTA, 2007, 592 (02) :121-131

[4]

Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography [J].

Chen, X. J. ;

Guo, B. L. ;

Li, S. P. ;

Zhang, Q. W. ;

Tu, P. F. ;

Wang, Y. T. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1163 (1-2) :96-104

[5]

Critical value determination on similarity of fingerprints [J].

Fang, Kai-Tai ;

Liang, Yi-Zeng ;

Yin, Xiao-lin ;

Chan, Kelvin ;

Lu, Guang-Hua .

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS, 2006, 82 (1-2) :236-240

[6]

FLAVONOIDS FROM EPIMEDIUM-KOREANUM [J].

KANG, SS ;

KANG, YJ ;

LEE, MW .

JOURNAL OF NATURAL PRODUCTS, 1991, 54 (02) :542-546

[7]

Chromatographic behaviour of steroidal saponins studied by high-performance liquid chromatography-mass spectrometry [J].

Kite, Geoffrey C. ;

Porter, Elaine A. ;

Simmonds, Monique S. J. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1148 (02) :177-183

[8]

Narrowbore high performance liquid chromatography of berberine and palmatine in crude drugs and pharmaceuticals with ion-pair extraction using cobalt thiocyanate reagent [J].

Lee, HS ;

Eom, YE ;

Eom, DO .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 21 (01) :59-63

[9]

Erxian Tang - Introduction of a Chinese herbal formula, clinical practice, and experimental studies [J].

Li Jie-jia ;

Li, Jack Thomas ;

Fu Jian-ping .

CHINESE JOURNAL OF INTEGRATIVE MEDICINE, 2007, 13 (01) :67-73

[10]

The cytoprotective effect of inulin-type hexasaccharide extracted from Morinda officinalis on PC12 cells against the lesion induced by corticosterone [J].

Li, YF ;

Liu, YQ ;

Yang, M ;

Wang, HL ;

Huang, WC ;

Zhao, YM ;

Luo, ZP .

LIFE SCIENCES, 2004, 75 (13) :1531-1538

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