Using a Commercial DiversiLab Semiautomated Repetitive Sequence-Based PCR Typing Technique for Identification of Escherichia coli Clone ST131 Producing CTX-M-15

被引：48

作者：

Pitout, Johann D. D. ^{[1
,2
,4
]}

Campbell, Lorraine ^{[1
]}

Church, Deirdre L. ^{[1
,2
,3
]}

Wang, Pauline W. ^{[5
]}

Guttman, David S. ^{[5
]}

Gregson, Daniel B. ^{[1
,2
,3
]}

机构：

[1] Calgary Lab Serv, Div Microbiol, Calgary, AB T2L 2K8, Canada

[2] Univ Calgary, Dept Pathol & Lab Med, Calgary, AB, Canada

[3] Univ Calgary, Dept Med, Calgary, AB, Canada

[4] Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada

[5] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 04期

关键词：

FIELD GEL-ELECTROPHORESIS; BETA-LACTAMASE; EMERGENCE; STRAIN;

D O I：

10.1128/JCM.02265-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A study was designed to evaluate the ability of the DiversiLab fingerprinting kit, a type of repetitive element PCR (rep-PCR), to identify Escherichia coli clone ST131 producing beta-lactamase CTX-M-15. A set of 53 nonduplicate isolates of extended-spectrum beta-lactamase-producing E. coli underwent rep-PCR, pulsed-field gel electrophoresis, and multilocus sequence typing. The DiversiLab system successfully identified E. coli clone ST131 producing CTX-M-15 and provides a simple standardized typing protocol for monitoring the spread of this clone.

引用

页码：1212 / 1215

页数：4

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