In vivo evaluation of the context sequence of the translation initiation codon in plants

被引：72

作者：

Lukaszewicz, M

Feuermann, M

Jérouville, B

Stas, A

Boutry, M

机构：

[1] Univ Catholique Louvain, Unite Biochim Physiol, B-1348 Louvain, Belgium

[2] Univ Wroclaw, Inst Microbiol, PL-51148 Wroclaw, Poland

来源：

PLANT SCIENCE | 2000年 / 154卷 / 01期

关键词：

consensus AUG context; dicot; gymnosperm; monocot; plant; translation context; translation initiation;

D O I：

10.1016/S0168-9452(00)00195-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Statistical analysis of the AUG initiation codon context in several plant organisms identified a nucleotide preference in some positions around the AUG. Sixteen AUG contexts were studied using transient expression in tobacco, maize and Norway spruce. Besides the importance of A or G at position -3, we revealed the role of positions - 2, - 1 for which AA or CC were found to be the best for tobacco and maize, respectively. GC (positions + 4, + 5) were also found to be important in both tobacco and maize. Finally, we identified a variation in context efficiency according to cell type, since A was better than G at position - 3 in tobacco leaf protoplasts, while both nucleotides were equally efficient in tobacco suspension cells. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：89 / 98

页数：10

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