Qualitative and quantitative analysis of serum immunoglobulins of four Antarctic fish species

被引：34

作者：

Scapigliati, G ^{[1
]}

Chausson, F ^{[1
]}

Cooper, EL ^{[1
]}

Scalia, D ^{[1
]}

Mazzini, M ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90024

来源：

POLAR BIOLOGY | 1997年 / 18卷 / 03期

关键词：

D O I：

10.1007/s003000050177

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Immunoglobulins from the Antarctic fish species Trematomus bernacchii, Trematomus hansoni, Trematomus newnesi, and Chionodraco hamatus were analysed in whole serum and after purification by affinity chromatography on protein A-sepharose. Using SDS-PAGE, the apparent masses of the heavy and light chains were, respectively, 83.5 kDa and 27.5 kDa for T. bernacchii, 83.5 kDa and 27 kDa for T. hansoni, 81 kDa and 27.5 kDa for T. newnesi, and 74.5 kDa and 30 kDa for C. hamatus. It was not possible to purify immunoglobulins from T. newnesi due to their low concentration in serum. Heterogeneity in mass of both heavy and light chains was observed in all species. By using a polyclonal antibody raised against sea bass immunoglobulins, cross-reactivity was observed with heavy and light chains of all species. With this antibody, an indirect enzyme-linked immunosorbent assay (ELISA) was developed and results showed the relative immunoglobulin concentration in sera of the Antarctic fish species considered? using as standard sea bass immunoglobulins.

引用

页码：209 / 213

页数：5

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