Chitosan sponges as tissue engineering scaffolds for bone formation

被引：259

作者：

Seol, YJ

Lee, JY

Park, YJ

Lee, YM

Young-Ku

Rhyu, IC

Lee, SJ

Han, SB

Chung, CP

机构：

[1] Seoul Natl Univ, Coll Dent, Dept Periodontol, Seoul 110749, South Korea

[2] Seoul Natl Univ, Coll Dent, Dept Craniomaxillofacial Reconstruct Sci, Seoul 110749, South Korea

[3] Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea

[4] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Periodontol, Seoul 135710, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 13期

关键词：

bone formation; chitosan sponges; osteoblast; tissue engineering;

D O I：

10.1023/B:BILE.0000032962.79531.fd

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100 - 200 mum pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) ( day 1), 11 x 10(6) ( day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 ( day 1), 40 (day 28) and 48 (day 56) mug per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

引用

页码：1037 / 1041

页数：5

共 13 条

[1] FACTORS THAT PROMOTE PROGRESSIVE DEVELOPMENT OF THE OSTEOBLAST PHENOTYPE IN CULTURED FETAL-RAT CALVARIA CELLS [J].