cDNA cloning, sequencing, and differential expression of a heart-type fatty acid-binding protein in the mummichog (Fundulus heteroclitus)

被引：11

作者：

Bain, LJ ^{[1
]}

机构：

[1] Univ Texas, Dept Sci Biol, El Paso, TX 79968 USA

来源：

MARINE ENVIRONMENTAL RESEARCH | 2002年 / 54卷 / 3-5期

关键词：

heart fatty acid binding protein; mummichog; Fundulus heteroclitus; pyrene;

D O I：

10.1016/S0141-1136(02)00130-7

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A heart fatty acid-binding protein (H-FABP) cDNA was isolated from the liver of the mummichog (Fundulus heteroclitus) and the complete cDNA sequence and predicted amino acid sequence was determined. This cDNA binds to a mRNA product of 0.95 kb with the highest level of RNA expression in the male liver, gills, and gonads. Exposure of mummichogs to the polycyclic aromatic hydrocarbon (PAH) pyrene significantly down-regulated H-FABP expression at the highest concentration. This study provides evidence that environmentally relevant toxicants can modulate H-FABP expression. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：379 / 383

页数：5

共 10 条

[1] LIGAND PROTEIN ELECTROSTATIC INTERACTIONS GOVERN THE SPECIFICITY OF RETINOL-BINDING AND FATTY ACID-BINDING PROTEINS [J].