On-the-fly fluorescence lifetime determination with total emission detection in HPLC

被引：17

作者：

Dvorak, MA ^{[1
]}

Oswald, GA ^{[1
]}

VanBenthem, MH ^{[1
]}

Gillispie, GD ^{[1
]}

机构：

[1] N DAKOTA STATE UNIV,DEPT CHEM,FARGO,ND 58105

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 17期

关键词：

D O I：

10.1021/ac961095j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The entire fluorescence decay profile during HPLC elution has been directly measured on-the-fly in HPLC at higher sensitivity than in previous literature reports, The fluorescence is excited with the fourth harmonic (266 mn) of a pulsed Nd:YAG laser system and detected broadband with a photomultiplier tube and a digital storage oscilloscope. Detection limits in the range 1-10 ppb are found for several individual polycyclic aromatic hydrocarbons (PAHs) when the total time-integrated fluorescence is analysed. The chromatograms of PAH mixtures containing 8-10 species were lifetime analysed with a simple phase plane analysis, in which a single lifetime is determined from the fluorescence decay profile for each point on the chromatogram. The determination of lifetimes under coelution conditions is also illustrated and discussed.

引用

页码：3458 / 3464

页数：7

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