Cyclin/Cdk-dependent initiation of DNA replication in a human cell-free system

被引：268

作者：

Krude, T ^{[1
]}

Jackman, M ^{[1
]}

Pines, J ^{[1
]}

Laskey, RA ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 3EJ,ENGLAND

来源：

CELL | 1997年 / 88卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S0092-8674(00)81863-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a cell-free system from HeLa cells that initiates DNA replication under cell cycle control. G1 but not G2 phase nuclei initiate replication when coincubated with S phase nuclei in cytosolic extracts from S phase but not from G1 or G2 phase HeLa cells. S phase nuclei or an S phase nuclear extract are required for the initiation of semiconservative DNA replication in G1 nuclei but not for elongation in S phase nuclei. S phase nuclear extract could be replaced by recombinant human cyclins A and E complexed to Cdk2 but not by Cdk2 alone or by human cyclin B1 complexed to CdcP. In S phase cytosol, cyclins A/Cdk2 and E/Cdk2 triggered initiation synergistically.

引用

页码：109 / 119

页数：11

共 47 条

[1] DEVELOPMENT OF BACULOVIRUS TRIPLE AND QUADRUPLE EXPRESSION VECTORS - COEXPRESSION OF 3 OR 4 BLUETONGUE VIRUS PROTEINS AND THE SYNTHESIS OF BLUETONGUE VIRUS-LIKE PARTICLES IN INSECT CELLS [J].