Generation of MHC-peptide tetramers: a new opportunity for dissecting T-cell immune responses

被引：12

作者：

Bousso, P ^{[1
]}

机构：

[1] Inst Pasteur, INSERM, U277, Unite Biol Mol Gene, F-75724 Paris 15, France

来源：

MICROBES AND INFECTION | 2000年 / 2卷 / 04期

关键词：

T-cell immunity; antigen-specific T cells; T-cell receptor;

D O I：

10.1016/S1286-4579(00)00324-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In the past few years, the technical breakthrough in generating MHC-peptide tetramers has revolutionized the analysis of T-cell responses. The major advantage of this technique over currently available methods is the ability of these tetramers to label T lymphocytes according to their antigenic specificity. The present review describes some technical aspects of tetramers generation and discusses some of the numerous possibilities opened up by this new technology. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

引用

收藏

页码：425 / 429

页数：5

相关论文

共 30 条

[1] Phenotypic analysis of antigen-specific T lymphocytes [J].

Altman, JD ;

Moss, PAH ;

Goulder, PJR ;

Barouch, DH ;

McHeyzerWilliams, MG ;

Bell, JI ;

McMichael, AJ ;

Davis, MM .

SCIENCE, 1996, 274 (5284) :94-96

[2] Initiation of signal transduction through the T cell receptor requires the peptide multivalent engagement of MHC ligands [J].

Boniface, JJ ;

Rabinowitz, JD ;

Wülfing, C ;

Hampl, J ;

Reich, Z ;

Altman, JD ;

Kantor, RM ;

Beeson, C ;

McConnell, HM ;

Davis, MM .

IMMUNITY, 1998, 9 (04) :459-466

[3] Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes [J].

Bousso, P ;

Michel, F ;

Pardigon, N ;

Bercovici, N ;

Liblau, R ;

Kourilsky, P ;

Abastado, JP .

IMMUNOLOGY LETTERS, 1997, 59 (02) :85-91

[4] The composition of a primary T cell response is largely determined by the timing of recruitment of individual T cell clones [J].

Bousso, P ;

Levraud, JP ;

Kourilsky, P ;

Abastado, JP .

JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (10) :1591-1600

[5] Individual variations in the murine T cell response to a specific peptide reflect variability in naive repertoires [J].

Bousso, P ;

Casrouge, A ;

Altman, JD ;

Haury, M ;

Kanellopoulos, J ;

Abastado, JP ;

Kourilsky, P .

IMMUNITY, 1998, 9 (02) :169-178

[6] Coordinate regulation of complex T cell populations responding to bacterial infection [J].

Busch, DH ;

Pilip, IM ;

Vijh, S ;

Pamer, EG .

IMMUNITY, 1998, 8 (03) :353-362

[7] T cell affinity maturation by selective expansion during infection [J].

Busch, DH ;

Pamer, EG .

JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (04) :701-709

[8] Evolution of a complex T cell receptor repertoire during primary and recall bacterial infection [J].

Busch, DH ;

Pilip, I ;

Pamer, EG .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (01) :61-70

[9] Direct visualization of antigen-specific CD8+ T cells during the primary immune response to Epstein-Barr virus in vivo [J].

Callan, MFC ;

Tan, L ;

Annels, N ;

Ogg, GS ;

Wilson, JDK ;

O'Callaghan, CA ;

Steven, N ;

McMichael, AJ ;

Rickinson, AB .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 187 (09) :1395-1402

[10] Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes [J].

Crawford, F ;

Kozono, H ;

White, J ;

Marrack, P ;

Kappler, J .

IMMUNITY, 1998, 8 (06) :675-682