Channel activators regulate ATP-sensitive potassium channel (KIR6.1) expression in chick cardiomyocytes

被引：23

作者：

Lu, CW ^{[1
]}

Halvorsen, SW ^{[1
]}

机构：

[1] SUNY BUFFALO,DEPT BIOCHEM PHARMACOL,AMHERST,NY 14260

来源：

FEBS LETTERS | 1997年 / 412卷 / 01期

关键词：

potassium channel; sulfonylurea; mRNA regulation; cardiac ion channel; pinacidil; ATP sensitive;

D O I：

10.1016/S0014-5793(97)00760-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP-sensitive potassium channels (K-ATP) are widely expressed and yet little is known about the mechanisms regulating their expression. Here we report that expression of chick heart Kir6.1 is regulated by channel activators. Activation of K-ATP with either ATP depletion or pinacidil, up-regulated Kir6.1 mRNA 1.8- to 2.4-fold in cultured ventricular myocytes as measured by competitive PCR. Pinacidil treatment also increased Kir6.1 protein as detected using an antibody to Kir6.1. Glibenclamide, a K-ATP inhibitor, completely blocked the pinacidil-induced increase in Kir6.1 levels. It appears that Kir6.1 is up-regulated by an unknown signal transduction pathway initiated by K-ATP opening. (C) 1997 Federation of European Biochemical Societies.

引用

页码：121 / 125

页数：5

共 33 条

[1] The sulphonylurea receptor confers diazoxide sensitivity on the inwardly rectifying K+ channel Kir6.1 expressed in human embryonic kidney cells [J].