Developmental changes in calcium channel types mediating central synaptic transmission

被引:218
作者
Iwasaki, S
Momiyama, A
Uchitel, OD
Takahashi, T [1 ]
机构
[1] Univ Tokyo, Fac Med, Dept Neurophysiol, Tokyo 1130033, Japan
[2] Natl Inst Physiol Sci, Lab Cerebral Struct, Okazaki, Aichi 4448585, Japan
[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Ciencias Biol, Lab Fisiol & Biol, RA-1428 Buenos Aires, DF, Argentina
关键词
N-type calcium channels; P/Q-type calcium channels; postnatal development; transmitter release; central synapse; slice;
D O I
10.1523/JNEUROSCI.20-01-00059.2000
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Multiple types of high-voltage-activated Ca2+ channels trigger neurotransmitter release at the mammalian central synapse. Among them, the omega-conotoxin GVIA-sensitive N-type channels and the omega-Aga-IVA-sensitive P/Q-type channels mediate fast synaptic transmission. However, at most central synapses, it is not known whether the contributions of different Ca2+ channel types to synaptic transmission remain stable throughout postnatal development. We have addressed this question by testing type-specific Ca2+ channel blockers at developing central synapses. Our results indicate that N-type channels contribute to thalamic and cerebellar IPSCs only transiently during early postnatal period and P/Q-type channels predominantly mediate mature synaptic transmission, as we reported previously at the brainstem auditory synapse formed by the calyx of Held. In fact, Ca2+ currents directly recorded from the auditory calyceal presynaptic terminal were identified as N-, P/Q-, and R-types at postnatal day 7 (P7) to P10 but became predominantly P/Q-type at P13. In contrast to thalamic and cerebellar IPSCs and brainstem auditory EPSCs, N- type Ca2+ channels persistently contribute to cerebral cortical EPSCs and spinal IPSCs throughout postnatal months. Thus, in adult animals, synaptic transmission is predominantly mediated by P/Q-type channels at a subset of synapses and mediated synergistically by multiple types of Ca2+ channels at other synapses.
引用
收藏
页码:59 / 65
页数:7
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