Pharmacologic overview of systemic chlorogenic acid therapy on experimental wound healing

被引:54
作者
Bagdas, Deniz [1 ]
Gul, Nihal Yasar [2 ]
Topal, Ayse [2 ]
Tas, Sibel [3 ]
Ozyigit, Musa Ozgur [4 ]
Cinkilic, Nilufer [3 ]
Gul, Zulfiye [5 ]
Etoz, Betul Cam [6 ]
Ziyanok, Sedef [3 ]
Inan, Sevda [4 ]
Turacozen, Ozge [3 ]
Gurun, Mine Sibel [5 ]
机构
[1] Uludag Univ, Expt Anim Breeding & Res Ctr, Fac Med, Bursa, Turkey
[2] Uludag Univ, Dept Surg, Fac Vet Med, Bursa, Turkey
[3] Uludag Univ, Fac Sci & Arts, Dept Biol, Bursa, Turkey
[4] Uludag Univ, Dept Pathol, Fac Vet Med, Bursa, Turkey
[5] Uludag Univ, Dept Pharmacol, Fac Med, Bursa, Turkey
[6] Uludag Univ, Dept Physiol, Fac Med, Bursa, Turkey
关键词
Antioxidant; Chlorogenic acid; Hydroxyproline; Matrix metalloproteinase-9; Tissue inhibitor-2; Wound; CAFFEIC ACID; NITRIC-OXIDE; MATRIX METALLOPROTEINASES; DIFFERENTIAL EXPRESSION; PROOXIDANT ACTIVITIES; TOPICAL APPLICATION; OXIDATIVE STRESS; PHENETHYL ESTER; INSTANT COFFEE; DNA-DAMAGE;
D O I
10.1007/s00210-014-1034-9
中图分类号
R9 [药学];
学科分类号
100702 [药剂学];
摘要
Chlorogenic acid (CGA) is a well-known natural antioxidant in human diet. To understand the effects of CGA on wound healing by enhancing antioxidant defense in the body, the present study sought to investigate the potential role of systemic CGA therapy on wound healing and oxidative stress markers of the skin. We also aimed to understand whether chronic CGA treatment has side effects on pivotal organs or rat bone marrow during therapy. Full-thickness experimental wounds were created on the backs of rats. CGA (25, 50, 100, 200 mg/kg) or vehicle was administered intraperitoneally for 15 days. All rats were sacrificed on the 16th day. Biochemical, histopathological, and immunohistochemical examinations were performed. Possible side effects were also investigated. The results suggested that CGA accelerated wound healing in a dose-dependent manner. CGA enhanced hydroxyproline content, decreased malondialdehyde and nitric oxide levels. and elevated reduced glutathione, superoxide dismutase, and catalase levels in wound tissues. Epithelialization, angiogenesis, fibroblast proliferation, and collagen formation increased by CGA while polymorph nuclear leukocytes infiltration decreased. CGA modulated matrix metalloproteinase-9 and tissue inhibitor-2 expression in biopsies. Otherwise, high dose of CGA increased lipid peroxidation of liver and kidney without affecting the heart and muscle samples. Chronic CGA increased micronuclei formation and induced cytotoxicity in the bone marrow. In conclusion, systemic CGA has beneficial effects in improving wound repair. Antioxidant, free radical scavenger, angiogenesis, and anti-inflammatory effects of CGA may ameliorate wound healing. High dose of CGA may induce side effects. In light of these observations, CGA supplementation or dietary CGA may have benefit on wound healing.
引用
收藏
页码:1101 / 1116
页数:16
相关论文
共 73 条
[2]
Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method [J].
Akkol, Esra Kupeli ;
Acikara, Ozlem Bahadir ;
Suntar, Ipek ;
Citoglu, Gulcin Saltan ;
Keles, Hikmet ;
Ergene, Burcin .
JOURNAL OF ETHNOPHARMACOLOGY, 2011, 137 (02) :1018-1027
[3]
ALBINA JE, 1990, J IMMUNOL, V144, P3877
[4]
[Anonymous], 2012, NUTR WELL BEING HLTH
[5]
INVOLVEMENT OF LIPID-PEROXIDATION IN NECROSIS OF SKIN FLAPS AND ITS SUPPRESSION BY ELLAGIC ACID [J].
ASHOORI, F ;
SUZUKI, S ;
ZHOU, JH ;
ISSHIKI, N ;
MIYACHI, Y .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1994, 94 (07) :1027-1037
[6]
Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties [J].
Azam, S ;
Hadi, N ;
Khan, NU ;
Hadi, SM .
TOXICOLOGY IN VITRO, 2004, 18 (05) :555-561
[7]
Absorption of chlorogenic acid and caffeic acid in rats after oral administration [J].
Azuma, K ;
Ippoushi, K ;
Nakayama, M ;
Ito, H ;
Higashio, H ;
Terao, J .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (11) :5496-5500
[8]
Effects of Systemic Chlorogenic Acid on Random-Pattern Dorsal Skin Flap Survival in Diabetic Rats [J].
Bagdas, Deniz ;
Etoz, Betul Cam ;
Ozturkoglu, Sevda Inan ;
Cinkilic, Nilufer ;
Ozyigit, Musa Ozgur ;
Gul, Zulfiye ;
Buyukcoskun, Naciye Isbil ;
Ozluk, Kasim ;
Gurun, Mine Sibel .
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2014, 37 (03) :361-370
[9]
Antihyperalgesic activity of chlorogenic acid in experimental neuropathic pain [J].
Bagdas, Deniz ;
Cinkilic, Nilufer ;
Ozboluk, Hasret Yucel ;
Ozyigit, Musa Ozgur ;
Gurun, Mine Sibel .
JOURNAL OF NATURAL MEDICINES, 2013, 67 (04) :698-704
[10]
Exogenous antioxidants-Double-edged swords in cellular redox state Health beneficial effects at physiologic doses versus deleterious effects at high doses [J].
Bouayed, Jaouad ;
Bohn, Torsten .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2010, 3 (04) :228-237