GnRH systems in masu salmon and barfin flounder

被引:3
作者
Amano, M [1 ]
Okubo, K
Yamanome, T
Oka, Y
Kitamura, S
Ikuta, K
Takahashi, A
Aida, K
Yamamori, K
机构
[1] Kitasato Univ, Sch Fisheries Sci, Ofunato, Iwate 0220101, Japan
[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Tokyo 1138657, Japan
[3] Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan
[4] Iwate Fisheries Technol Ctr, Kamaishi, Iwate 0260001, Japan
[5] Univ Tokyo, Grad Sch Sci, Misaki Marine Biol Stn, Kanagawa 2380225, Japan
[6] Natl Res Inst Aquaculture, Nikko Branch, Nikko, Tochigi 3211661, Japan
关键词
barfin flounder; immunocytochemistry; in situ hybridization; GnRH; masu salmon; radioimmunoassay;
D O I
10.1023/B:FISH.0000030463.33250.d2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multiple forms of the gonadotropin-releasing hormone ( GnRH) exist in teleost fish. A salmonid fish, masu salmon Oncorhynchus masou has salmon GnRH ( sGnRH) and chicken GnRH-II (cGnRH-II). sGnRH neurons were scattered from the olfactory nerve through the ventral telencephalon (VT) and the preoptic area (POA). sGnRH but not cGnRH-II was detected in the pituitary. sGnRH mRNA levels in the VT and the POA increased during gonadal maturation, suggesting that sGnRH neurons in these areas are involved in gonadal maturation. sGnRH neurons were first detected in a cluster near the olfactory epithelium 40 days after fertilization. sGnRH neurons were not detected in the brain by the olfactory epithelia lesion, suggesting that sGnRH neurons are derived from the olfactory epithelium. A pleuronectiform fish, barfin flounder Verasper moseri has sGnRH, cGnRH-II and seabream GnRH (sbGnRH). sGnRH and cGnRH-II-immunoreactive fibers were observed throughout the brain, but not in the pituitary. sbGnRH neurons were located in the POA and sent fibers to the pituitary, indicating that sbGnRH is involved in GTH secretion. Judging from the location of neuronal somata and their projections, it is indicated that three GnRH systems exist in the barfin flounder; the TN-, the MT- and the POA-GnRH system. However, in masu salmon, clear anatomical identification of the TN- and the POA-GnRH system is difficult, because the GnRH neurons located in the ventral forebrain are consecutive and the GnRH form produced in these neurons is the same ( sGnRH). Thus, it is suggested in masu salmon that sGnRH neurons are derived from the olfactory epithelium, migrate into the brain and play different roles according to the location in the brain.
引用
收藏
页码:19 / 22
页数:4
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