Probing carotenoid biosynthesis in developing seed coats of Bixa orellana (Bixaceae) through expressed sequence tag analysis

被引：30

作者：

Jako, C ^{[1
]}

Coutu, C ^{[1
]}

Roewer, I ^{[1
]}

Reed, DW ^{[1
]}

Pelcher, LE ^{[1
]}

Covello, PS ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

来源：

PLANT SCIENCE | 2002年 / 163卷 / 01期

关键词：

expressed sequence tag; Bixa orellana; carotenoid; bixin; dioxygenase; carboxyl methyltransferase;

D O I：

10.1016/S0168-9452(02)00083-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bixin is a commercially important diapocarotenoid derived from the seed coats of the tropical shrub Bixa orellana. Based on available chemical data and by analogy to abscisic acid biosynthesis, a biosynthetic pathway for bixin starting from lycopene is proposed. This putative pathway was investigated through expressed sequence tag (EST) analysis using a subtracted cDNA library. This analysis identified ESTs corresponding to most of the enzymes of the carotenoid biosynthesis pathway including the 1-deoxy-D-xylulose 5-phosphate pathway of plastids. ESTs corresponding to a number of genes encoding dioxygenases, aldehyde dehydrogenases and carboxyl methyltransferases were also identified. These represent candidate enzymes for the last stage of the bixin biosynthetic pathway. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：141 / 145

页数：5

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