A simple and rapid method for the preparation of a cell-free extract with CCAAT-binding activity from filamentous fungi

被引：9

作者：

Kato, M ^{[1
]}

Hayashi, K ^{[1
]}

Kobayashi, T ^{[1
]}

Tsukagoshi, N ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Biol Mechanisms & Funct, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 02期

关键词：

filamentous fungi; CCAAT-binding protein; cell free extracts; electrophoretic mobility shift assays;

D O I：

10.1271/bbb.64.455

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride.

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收藏

页码：455 / 457

页数：3

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